现代仪器与医疗
現代儀器與醫療
현대의기여의료
Modern Instrument and Medical Treatment
2014年
3期
1-3
,共3页
原艳明%王仲元%王心静%程小星%安慧茹%翟守恒%张玲莉%吕双梅
原豔明%王仲元%王心靜%程小星%安慧茹%翟守恆%張玲莉%呂雙梅
원염명%왕중원%왕심정%정소성%안혜여%적수항%장령리%려쌍매
白细胞介素-22%结核性胸膜炎%恶性胸水%鉴别诊断
白細胞介素-22%結覈性胸膜炎%噁性胸水%鑒彆診斷
백세포개소-22%결핵성흉막염%악성흉수%감별진단
Interleukin-22%Tuberculous pleurisy%Malignant pleural effusions%differential diagnosis
目的：研究胸液单个核细胞经结核分枝杆菌特异性抗原肽刺激后表达的白细胞介素-22（Interleukin-22，IL-22）浓度测定在结核性与恶性胸腔积液鉴别诊断中的价值。方法：将确诊的52例结核性胸膜炎和35例恶性胸腔积液患者胸液单个核细胞分离后，使用结核分枝杆菌特异抗原肽（early secreted antigenic target-6，ESAT-6）、（culture filtrate protein-10，CFP-10）刺激培养，测定细胞培养液上清中IL-22和干扰素-γ（Interferon-γ，IFN-γ）浓度，对结果进行统计学分析。结果：结核性胸膜炎组I L-22浓度明显高于恶性胸液组( P<0.01)。R O C曲线显示I L-22用于结核性胸膜炎的诊断敏感性、特异性和准确性分别为90.38%、80.56%和85.23%，其诊断效能与IFN-γ相当(P>0.05)。结论：结核分枝杆菌抗原特异反应性IL-22测定可作为对结核性胸膜炎诊断的重要参考指标。
目的：研究胸液單箇覈細胞經結覈分枝桿菌特異性抗原肽刺激後錶達的白細胞介素-22（Interleukin-22，IL-22）濃度測定在結覈性與噁性胸腔積液鑒彆診斷中的價值。方法：將確診的52例結覈性胸膜炎和35例噁性胸腔積液患者胸液單箇覈細胞分離後，使用結覈分枝桿菌特異抗原肽（early secreted antigenic target-6，ESAT-6）、（culture filtrate protein-10，CFP-10）刺激培養，測定細胞培養液上清中IL-22和榦擾素-γ（Interferon-γ，IFN-γ）濃度，對結果進行統計學分析。結果：結覈性胸膜炎組I L-22濃度明顯高于噁性胸液組( P<0.01)。R O C麯線顯示I L-22用于結覈性胸膜炎的診斷敏感性、特異性和準確性分彆為90.38%、80.56%和85.23%，其診斷效能與IFN-γ相噹(P>0.05)。結論：結覈分枝桿菌抗原特異反應性IL-22測定可作為對結覈性胸膜炎診斷的重要參攷指標。
목적：연구흉액단개핵세포경결핵분지간균특이성항원태자격후표체적백세포개소-22（Interleukin-22，IL-22）농도측정재결핵성여악성흉강적액감별진단중적개치。방법：장학진적52례결핵성흉막염화35례악성흉강적액환자흉액단개핵세포분리후，사용결핵분지간균특이항원태（early secreted antigenic target-6，ESAT-6）、（culture filtrate protein-10，CFP-10）자격배양，측정세포배양액상청중IL-22화간우소-γ（Interferon-γ，IFN-γ）농도，대결과진행통계학분석。결과：결핵성흉막염조I L-22농도명현고우악성흉액조( P<0.01)。R O C곡선현시I L-22용우결핵성흉막염적진단민감성、특이성화준학성분별위90.38%、80.56%화85.23%，기진단효능여IFN-γ상당(P>0.05)。결론：결핵분지간균항원특이반응성IL-22측정가작위대결핵성흉막염진단적중요삼고지표。
Objective:To research the diagnosis value of the measurement of the concentration of Interleukin-22(IL-22)generated by the pleural fluid mononuclear cells(PFMCs)stimulated by specific MTB antigen peptides in tuberculous Pleurisy effusions(TPE)and malignant pleural effusions(MPE).Methods: The pleural fluid mononuclear cells(PFMCs)of 52 cases of TPE and 35 cases of MPE were separated and cultivated with the stimulation of specific MTB antigen peptides(early secreted antigenic target-6,ESAT-6)、(culture filtrate protein-10,CFP-10), the concentration of IL-22 and Interferon-γ(IFN-γ)of the PFMCs culture lfuid supernatant were measured and the results were statistically analyzed.Results:The concentration of IL-22 in TPE group was significantly higher than MPE group(P<0.01).ROC curve showed the sensibility、speciifcity and the accuracy of IL-22 were 90.38%、80.56% and 85.23% when used in the diagnosis of TPE, the diagnose efifciency was equal to IFN-γ(P>0.05).Conclusion: The measurement of the Mtb-antigen-speciifc IL-22 can be regarded as an important reference index in the diagnosis of TPE.
