CAJ | 학술논문

目的：探讨17α-己酸羟孕酮对外周血单个核细胞抵抗地塞米松的逆转作用。方法分离健康男性外周血单个核细胞，利用白介素2（IL-2）和IL-4以及植物血凝素刺激外周血单个核细胞，建立地塞米松抵抗的炎症细胞实验模型；单用地塞米松和地塞米松与17α-己酸羟孕酮联合应用于该模型，酶联免疫吸附法检测细胞的IL-2值的改变。结果地塞米松对植物血凝素诱导人外周血产生的IL-2呈现显著的浓度依赖性的抑制作用；用IL-2（13 ng · mL -1）和IL-4（6．5 ng · mL -1）刺激，再用植物血凝素（15μg · mL -1）刺激外周血单个核细胞可以作为评价地塞米松抵抗的细胞实验模型；在17α-己酸羟孕酮的浓度为10-11，10-10，10-7和10-5 mol · L -1时，可显著提高地塞米松对人外周血产生的IL-2的抑制率，降低地塞米松的IC50（半数抑制浓度）值至少100倍以上。结论17α-己酸羟孕酮逆转外周血单个核细胞对地塞米松的抵抗，可作为地塞米松等糖皮质激素的增敏剂。
목적：탐토17α-기산간잉동대외주혈단개핵세포저항지새미송적역전작용。방법분리건강남성외주혈단개핵세포，이용백개소2（IL-2）화IL-4이급식물혈응소자격외주혈단개핵세포，건입지새미송저항적염증세포실험모형；단용지새미송화지새미송여17α-기산간잉동연합응용우해모형，매련면역흡부법검측세포적IL-2치적개변。결과지새미송대식물혈응소유도인외주혈산생적IL-2정현현저적농도의뢰성적억제작용；용IL-2（13 ng · mL -1）화IL-4（6．5 ng · mL -1）자격，재용식물혈응소（15μg · mL -1）자격외주혈단개핵세포가이작위평개지새미송저항적세포실험모형；재17α-기산간잉동적농도위10-11，10-10，10-7화10-5 mol · L -1시，가현저제고지새미송대인외주혈산생적IL-2적억제솔，강저지새미송적IC50（반수억제농도）치지소100배이상。결론17α-기산간잉동역전외주혈단개핵세포대지새미송적저항，가작위지새미송등당피질격소적증민제。
Objective To investigate the reversal effect of 17α-hydroxyprogesterone caproate on dexamethasone resistant in human peripheral blood mononuclear cells .Methods Peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers and were stimulated with interleukin-2(IL-2) IL-2 ,IL-4 and phytohemagglutinin to establish a cell study model of dexamethasone (DEX) resistance .By using the model ,PBMCs were stimulated with DEX alone or in combination with 17α-hydroxyprogesterone caproate(17HPC) ,and the IL-2 levels were quantified by enzyme linked immunosorbent assay (ELISA) .Results The production of IL-2 was inhibited by DEX in a significant ,dose-dependent manner .Following the pretreatment with IL-2(13 ng · mL -1 ) and IL-4(6 .5 ng · mL -1 ) ,PBMCs were stimulated with phytohemagglutinin and the experimental model of DEX resistance was estab-lished .The inhibition effects of DEX on the production of IL-2 were enhanced significantly by 17HPC .The IC50 values(50% in-hibitory concentrations) of DEX were decreased at least 100-fold compared to the control (without 17HPC) caused by 10-11 , 10-10 ,10-7 and 10-5 mol · L -1 17HPC .Conclusion 17α-hydroxyprogesterone caproate can reverse dexamethasone resistance in human peripheral blood mononuclear cells and it may be used as a glucocorticoid sensitizer .