西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
3期
255-257
,共3页
黄萍%高琳%司马磊%饶雅琨%刘霞%郑妍
黃萍%高琳%司馬磊%饒雅琨%劉霞%鄭妍
황평%고림%사마뢰%요아곤%류하%정연
四环素片%高效液相色谱法%含量测定
四環素片%高效液相色譜法%含量測定
사배소편%고효액상색보법%함량측정
Tetracycline Tablets%HPLC%determination
目的:建立高效液相色谱(HPLC)法测定四环素片的含量。方法采用Kromasil C18(250 mm ×4.6 mm ,5μm)色谱柱,以0.1 mol · L -1磷酸氢二铵-乙腈(84∶16)为流动相,柱温为35℃,流速为1.0 mL · min-1,检测波长为280 nm。结果四环素质量浓度在0.0240~0.7697 mg · mL -1范围内呈良好的线性关系,r=1.000(n=6),平均回收率为100.4%,RSD为0.8%(n=3)。结论该方法操作简便、准确、重复性好,可用于四环素片的含量测定。
目的:建立高效液相色譜(HPLC)法測定四環素片的含量。方法採用Kromasil C18(250 mm ×4.6 mm ,5μm)色譜柱,以0.1 mol · L -1燐痠氫二銨-乙腈(84∶16)為流動相,柱溫為35℃,流速為1.0 mL · min-1,檢測波長為280 nm。結果四環素質量濃度在0.0240~0.7697 mg · mL -1範圍內呈良好的線性關繫,r=1.000(n=6),平均迴收率為100.4%,RSD為0.8%(n=3)。結論該方法操作簡便、準確、重複性好,可用于四環素片的含量測定。
목적:건립고효액상색보(HPLC)법측정사배소편적함량。방법채용Kromasil C18(250 mm ×4.6 mm ,5μm)색보주,이0.1 mol · L -1린산경이안-을정(84∶16)위류동상,주온위35℃,류속위1.0 mL · min-1,검측파장위280 nm。결과사배소질량농도재0.0240~0.7697 mg · mL -1범위내정량호적선성관계,r=1.000(n=6),평균회수솔위100.4%,RSD위0.8%(n=3)。결론해방법조작간편、준학、중복성호,가용우사배소편적함량측정。
Objective To establish an improved HPLC method for the determination of Tetracycline Tablets .Methods The separa-tion was performed on a Kromasil C18 (250 mm × 4 .6 mm ,5 μm) column ,with the mobile phase of 0 .1 mol · L -1 diammonium hydrogen phosphate-acetonitriles(84∶16) at a flow rate of 1 .0 mL · min-1 .The column temperature was set at 35 ℃ ,and the detection wavelength was 280 nm .Results Tetracycline presented a good linear correlation in the range of 0 .024 0-0 .769 7 mg · mL -1 (r=1 .000) ,the limit of detection was 21 ng · mL -1 ,and the average recovery was 100 .4% ,with RSD of 0 .8% (n=9) .Conclusion This method was simple ,accurate ,reproducible ,and suitable for the quality control of Tetracycline Tablets .