CAJ | 학술논문

目的：建立维血宁的高效液相色谱指纹图谱。方法采用HPLC法，以虎杖苷、大黄素-8-O-β-D葡萄糖苷、大黄素甲醚-8-O-β-D葡萄糖、大黄素和大黄素甲醚为内参照物，色谱柱为AccurasilC18（250mm×4．6mm，5μm），流动相为乙腈-1mL·L-1磷酸水（梯度洗脱），检测波长为280nm，流速为1．0mL·min-1，柱温为30℃，分析时间为90min。结果在HPLC指纹图谱中确定了5个主要共有峰，建立了维血宁的对照图谱。相似度比较结果显示，10批维血宁的对照图谱相似度均大于0．900。结论采用HPLC 方法建立维血宁指纹图谱，方法稳定、可靠、简便，色谱图展现的各峰分离度较好，特征明显，可作为维血宁真伪鉴别标准，为维血宁的质量评价提供参考依据。
목적：건립유혈저적고효액상색보지문도보。방법채용HPLC법，이호장감、대황소-8-O-β-D포도당감、대황소갑미-8-O-β-D포도당、대황소화대황소갑미위내삼조물，색보주위AccurasilC18（250mm×4．6mm，5μm），류동상위을정-1mL·L-1린산수（제도세탈），검측파장위280nm，류속위1．0mL·min-1，주온위30℃，분석시간위90min。결과재HPLC지문도보중학정료5개주요공유봉，건립료유혈저적대조도보。상사도비교결과현시，10비유혈저적대조도보상사도균대우0．900。결론채용HPLC 방법건립유혈저지문도보，방법은정、가고、간편，색보도전현적각봉분리도교호，특정명현，가작위유혈저진위감별표준，위유혈저적질량평개제공삼고의거。
Objective To establish a fingerprint analytical method of Weixuening .Methods By using polydatin ,emodin-8-O-β-D-glu-coside ,physcionas-8-O-β-D-glycosidase ,emodin and physcionas as reference substances ,the chromatographic separations were per-formed on an Accurasil C18 (250 mm × 4 .6 mm ,5 μm) column ,the column temperature was maintained at 30 ℃ ,a linear elution of 1 mL · L -1 phosphoric acid aqueous solution-acetonitrile was adopted at a flow rate of 1 mL · min-1 ,the UV detection wavelength was 280 nm and the analysis time was 90 min .Results There were five main common peaks in the fingerprint .The similarity of samples was all over 0 .900 .Conclusion The HPLC method for establishing the fingerprint was stable ,reliable ,and simple ,and the obtained chromatograms showed good separation and obvious peak characteristics ,which can be used as the standard for the identi-fication of decoction pieces in Weixuening .