CAJ | 학술논문

目的：研究艾灸对大鼠创伤皮肤组织成纤维细胞和角化细胞生长因子（ KGF）表达的影响，探讨艾灸对大鼠皮肤创伤修复的作用。方法：建立大鼠背部皮肤创伤模型，采用随机数字表法，将24只大鼠分为治疗组和模型组。根据治疗时间的不同，又将每组分为1天、3天、7天共3个时间点，每个时间点4只大鼠。治疗组进行艾灸治疗，每次30 min，每日1次。以HE染色法检测1天、3天、7天3个时间点修复组织内成纤维细胞数；以免疫组化法检测1天、3天、7天等3个时间点修复组织成纤维细胞生长因子的表达情况。结果：大鼠创面愈合指数治疗组高于模型组（P＜0．05）。大鼠皮肤创面成纤维细胞数，7天治疗组皮肤创面成纤维细胞数高于模型组（P ＜0．05）；3天两组比较差异无统计学意义（P ＞0.05）。大鼠创面KGF表达情况，3天模型组KGF表达呈弱阳性，治疗组表达呈阳性；7天模型组KGF表达呈阳性，治疗组表达呈强阳性。结论：艾灸能促进大鼠皮肤创伤愈合，其机制可能与调节不同时间内创面组织成纤维细胞数和KGF含量有关。
목적：연구애구대대서창상피부조직성섬유세포화각화세포생장인자（ KGF）표체적영향，탐토애구대대서피부창상수복적작용。방법：건립대서배부피부창상모형，채용수궤수자표법，장24지대서분위치료조화모형조。근거치료시간적불동，우장매조분위1천、3천、7천공3개시간점，매개시간점4지대서。치료조진행애구치료，매차30 min，매일1차。이HE염색법검측1천、3천、7천3개시간점수복조직내성섬유세포수；이면역조화법검측1천、3천、7천등3개시간점수복조직성섬유세포생장인자적표체정황。결과：대서창면유합지수치료조고우모형조（P＜0．05）。대서피부창면성섬유세포수，7천치료조피부창면성섬유세포수고우모형조（P ＜0．05）；3천량조비교차이무통계학의의（P ＞0.05）。대서창면KGF표체정황，3천모형조KGF표체정약양성，치료조표체정양성；7천모형조KGF표체정양성，치료조표체정강양성。결론：애구능촉진대서피부창상유합，기궤제가능여조절불동시간내창면조직성섬유세포수화KGF함량유관。
Objective:To observe the effect of moxibustion on the number of fibroblasts and the expression of keratinocyte growth factor ( KGF) in the cutaneous wound rats so as to study its effect on rats cutaneous wound healing .Methods:24 SD rats were made full -thickness cutaneous wound at the back .All the SD rats were randomly divided into model ( n=12 ) groups and treatment ( n=12 ) groups .According to different treatment time, the treatment and model groups were randomly divided into three sub -groups by 1 d, 3 d, 7 d, three time points, each sub-group 4 SD rats.For the treatment groups, moxibustion was applied to local raw surface tissue of cutaneous wound for 30 min, once daily.The number of fibroblasts in the raw surface tissue was coun-ted under light microscope after H.E.staining and the keratinocyte growth factor (KGF) expressions of the raw surface tissue were detected by immunohistochemistry at the 1st, 3th, 7th treatment day.Results:The wound healing rate of the treatment groups were totally higher than that of the model groups , and there were statistical differences(P<0.05).Compared with the model group, the number of fibroblasts in the raw surface tissue were increased significantly in the 7 d sub-group of the treatment one(P<0.05);while there were no statisti-cal differences in the 3 d sub-group( P>0.05) .In regard to the KGF expression in the raw surface tissue , it was weakly positive in 3 d sub -model group, while positive in 3 d sub -treatment group.It was positive in the7 d sub -model group and strongly positive in the 7 d sub -treatment group.Conclusion:By regulating thenumber of fibroblasts and the expression of KGF , moxibustion can promote rats'cutaneous wound healing.