中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
4期
396-399,410
,共5页
骨髓间充质干细胞%miR-210%补肾活血
骨髓間充質榦細胞%miR-210%補腎活血
골수간충질간세포%miR-210%보신활혈
Bone mesenehymal stem cells%miR-210%Kidney-tonifying and blood circulation-promoting recipes
目的：研究补肾活血方促进骨髓间充质干细胞（Bone Mesenehymal Stem Cells ，BMSCs）成骨分化的分子机制。方法贴壁培养法分离纯化大鼠BMSCs，将培养成功的BMSCs分为空白组、成骨诱导组和中药组，培养7d、14d行矿化结节茜素红染色（Alizarin Red S，ARS），倒置显微镜观察矿化骨结节形成情况，采用real-time PCR检测miR-210的表达变化。结果与空白组比较，成骨诱导组和中药组BMSCs钙化结节数量明显增多，差异有非常显著的统计学意义（ P＜0.01）。在成骨诱导剂、补肾活血方水提液的作用下，与空白组比较，miR-210表达明显降低，且中药组较成骨诱导组更明显降低miR-210表达。结论补肾活血方通过降低miR-210表达促进BMSCs成骨分化。
目的：研究補腎活血方促進骨髓間充質榦細胞（Bone Mesenehymal Stem Cells ，BMSCs）成骨分化的分子機製。方法貼壁培養法分離純化大鼠BMSCs，將培養成功的BMSCs分為空白組、成骨誘導組和中藥組，培養7d、14d行礦化結節茜素紅染色（Alizarin Red S，ARS），倒置顯微鏡觀察礦化骨結節形成情況，採用real-time PCR檢測miR-210的錶達變化。結果與空白組比較，成骨誘導組和中藥組BMSCs鈣化結節數量明顯增多，差異有非常顯著的統計學意義（ P＜0.01）。在成骨誘導劑、補腎活血方水提液的作用下，與空白組比較，miR-210錶達明顯降低，且中藥組較成骨誘導組更明顯降低miR-210錶達。結論補腎活血方通過降低miR-210錶達促進BMSCs成骨分化。
목적：연구보신활혈방촉진골수간충질간세포（Bone Mesenehymal Stem Cells ，BMSCs）성골분화적분자궤제。방법첩벽배양법분리순화대서BMSCs，장배양성공적BMSCs분위공백조、성골유도조화중약조，배양7d、14d행광화결절천소홍염색（Alizarin Red S，ARS），도치현미경관찰광화골결절형성정황，채용real-time PCR검측miR-210적표체변화。결과여공백조비교，성골유도조화중약조BMSCs개화결절수량명현증다，차이유비상현저적통계학의의（ P＜0.01）。재성골유도제、보신활혈방수제액적작용하，여공백조비교，miR-210표체명현강저，차중약조교성골유도조경명현강저miR-210표체。결론보신활혈방통과강저miR-210표체촉진BMSCs성골분화。
Objective To investigate the mechanism of osteogenic differentiation of bone mesenehymal stem cells ( BMSCs) promoted by the kidney-tonifying and blood circulation-promoting recipes.Methods BMSCs of SD rats were isolated with adherent separation method and primarily cultured in vitro.Then BMSCs were divided into blank group, osteo-induced group, and Chinese medicine group.At the 7 th day and the 14 th day, the mineralized nodules were stained using Alizarin red statining ( ARS) . The formation of mineralized nodules was observed using a converted microscope.The expression of miR-210 was detected using real-time PCR.Results Compared with that in blank group, the number of mineralized nodules in osteo-induced group and Chinese medicine group increased significantly ( P <0.01 ) .After the intervention of osteogenic inducer and extraction of the kidney-tonifying and blood circulation-promoting recipes, the expression of miR-210 in both groups decreased significantly compared with that in blank group.And the expression in Chinese medicine group was much lower than that in osteo-induced group. Conclusion The kidney-tonifying and blood circulation-promoting recipes can promote the osteogenic differentiation of BMSCs by down-regulating the expression of miRNA-210.
