CAJ | 학술논문

目的：检测胰岛素抵抗（IR）和2型糖尿病（T2DM）大鼠的胰岛鼠抵抗情况、血清25-（OH）D3和1，25-（OH）2D3水平、腰椎和股骨骨密度（ BMD），探讨IR与2型糖尿病时血清维生素D3和骨密度变化中的意义。方法18月龄wistar大鼠30只，分为正常对照组（N组）、胰岛素抵抗组（I组）、糖尿病组（D组），正常血糖胰岛素钳夹技术（EICT）测定各组大鼠IR，葡萄糖输注速率（GIR）表示IR，放免法测定各组大鼠血25-（OH）D3和1，25-（OH）2D3水平，双能X线骨密度测量仪（DEXA）测定各组大鼠腰椎、股骨BMD。结果 D组和I组GIR相当，均显著低于N组（P＜0.01），I组1，25-（OH）2D3低于N组（P＜0.05），高于D组（P＜0.01），三组间25-（OH）D3无显著差异，I组腰椎、股骨BMD低于N照组，高于D组（P＜0.05）。结论 IR是2型糖尿病导致血清活性维生素D3降低和骨密度下降的重要病理生理基础。
목적：검측이도소저항（IR）화2형당뇨병（T2DM）대서적이도서저항정황、혈청25-（OH）D3화1，25-（OH）2D3수평、요추화고골골밀도（ BMD），탐토IR여2형당뇨병시혈청유생소D3화골밀도변화중적의의。방법18월령wistar대서30지，분위정상대조조（N조）、이도소저항조（I조）、당뇨병조（D조），정상혈당이도소겸협기술（EICT）측정각조대서IR，포도당수주속솔（GIR）표시IR，방면법측정각조대서혈25-（OH）D3화1，25-（OH）2D3수평，쌍능X선골밀도측량의（DEXA）측정각조대서요추、고골BMD。결과 D조화I조GIR상당，균현저저우N조（P＜0.01），I조1，25-（OH）2D3저우N조（P＜0.05），고우D조（P＜0.01），삼조간25-（OH）D3무현저차이，I조요추、고골BMD저우N조조，고우D조（P＜0.05）。결론 IR시2형당뇨병도치혈청활성유생소D3강저화골밀도하강적중요병리생리기출。
Objective To detect the insulin resistance (IR), the serum level of 25-(OH)2D3 and 1,25-(OH)2D3, and the bone mineral density (BMD) of the lumbar vertebrae and the femur in rats with type 2 diabetes mellitus (T2DM), and to investigate the effect of IR on the changes of serum 1,25-(OH)2D3and BMD in T2DM.Methods Thirty 18-month Wistar rats were randomly divided into normal control group (N), insulin resistance group (I), and diabetes group (D).IR of rats in each group was detected using EICT, expressed as GIR.The serum levels of 25-( OH) D3 and 1, 25-( OH)2D3 were measured using radioimmunoassay.BMD of the lumbar vertebrae and the femur was detected using dual energy X-ray absorptionmetry (DEXA). Results The level of GIR in D group and I group was almost the same, while both were significantly lower than that in N group (P<0.01).The serum level of 1,25-(OH)2D3in I group was lower than that in N group (P<0.05), while higher than that in D group (P<0.01).The serum level of 25-(OH)D3 showed no significant difference among 3 groups.BMD of the lumbar vertebrae and the femur in I group was lower than that in N group, while higher than that in D group (P<0.05).Conclusion IR is the vital pathophysiological factor for the decrease of serum 1,25-(OH)2D3and BMD in T2DM.