热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
4期
42-45
,共4页
刘巧莲%朱军%高建明%张世清%陈河龙%郑金龙%易克贤
劉巧蓮%硃軍%高建明%張世清%陳河龍%鄭金龍%易剋賢
류교련%주군%고건명%장세청%진하룡%정금룡%역극현
H.11648麻%快繁%MSSH
H.11648痳%快繁%MSSH
H.11648마%쾌번%MSSH
H.11648 hybrid sisal%rapid propagation%MS%SH
以剑麻H.11648为材料，研究不同外植体、培养基和激素对不定芽诱导及生根的影响。试验结果表明，以珠芽苗茎尖为最佳快繁材料，最佳消毒时间25 min，最适合的基本培养基为SH；最佳分化培养基为SH＋NAA 0.05 mg/L＋6-BA 4 mg/L，芽的分化率达到85%，同时可做增殖培养基，增殖平均倍数为18。分化芽在MS和SH两种培养基中添加NAA 0.1 mg/L＋6-BA 0.1 mg/L中均可生根，生根率为95%。
以劍痳H.11648為材料，研究不同外植體、培養基和激素對不定芽誘導及生根的影響。試驗結果錶明，以珠芽苗莖尖為最佳快繁材料，最佳消毒時間25 min，最適閤的基本培養基為SH；最佳分化培養基為SH＋NAA 0.05 mg/L＋6-BA 4 mg/L，芽的分化率達到85%，同時可做增殖培養基，增殖平均倍數為18。分化芽在MS和SH兩種培養基中添加NAA 0.1 mg/L＋6-BA 0.1 mg/L中均可生根，生根率為95%。
이검마H.11648위재료，연구불동외식체、배양기화격소대불정아유도급생근적영향。시험결과표명，이주아묘경첨위최가쾌번재료，최가소독시간25 min，최괄합적기본배양기위SH；최가분화배양기위SH＋NAA 0.05 mg/L＋6-BA 4 mg/L，아적분화솔체도85%，동시가주증식배양기，증식평균배수위18。분화아재MS화SH량충배양기중첨가NAA 0.1 mg/L＋6-BA 0.1 mg/L중균가생근，생근솔위95%。
The effects of explants and cultural factors on induction of adventitious shoots and plant regeneration of sisal H.11648 was studied. The results showed that the best explant for rapid propagation was stem tip of bead bud seedling, the suitable sterilization time was 25 min, and the most suitable basic culture medium was SH. The best medium for differentiation and proliferation was SH+ NAA0.05 mg/L+ 6-BA4 mg/L, bud differentiation rate reached 85%, and proliferation average was 18. Root could well developed in MS (SH)+NAA0.1 mg/L+6-BA0.1 mg/L and rooting rate was up to 95%.
