CAJ | 학술논문

以热研2号柱花草花药为外植体，研究不同植物生长调节剂、培养基以及低温预处理等对柱花草花药培养的影响，以期建立柱花草花药培养体系，获得再生植株，为柱花草倍性、基因组学及分子生物学研究提供材料。结果表明：花药4℃低温预处理24 h诱导效果较好； N6培养基对愈伤组织的诱导效果比MS培养基好；最佳愈伤组织诱导培养基组合为N6＋2,4-D 0.5 mg/L＋KT1.0 mg/L；分化培养的最佳组合为MS＋NAA 0.2 mg/L＋6-BA 3.0 mg/L。
이열연2호주화초화약위외식체，연구불동식물생장조절제、배양기이급저온예처리등대주화초화약배양적영향，이기건립주화초화약배양체계，획득재생식주，위주화초배성、기인조학급분자생물학연구제공재료。결과표명：화약4℃저온예처리24 h유도효과교호； N6배양기대유상조직적유도효과비MS배양기호；최가유상조직유도배양기조합위N6＋2,4-D 0.5 mg/L＋KT1.0 mg/L；분화배양적최가조합위MS＋NAA 0.2 mg/L＋6-BA 3.0 mg/L。
In order to establish an efficient anther culture system of Stylosanthes, get regenerate plantlet and provide basic materials for the research of ploidy, genomics and molecular biology of Stylosanthes. This experiment taking the anther of Stylosanthes guianensis cv, Reyan2 as explants to study the effect of plant growth regulator, medium and low temperature pretreatment on Stylosanthes anther culture. The results indicated that low temperature pretreatment for 24 h was better than other treatments. N6 medium supplemented with 2,4-D 0.5 mg/L, KT 1.0 mg/L was the best for induction of cullus. MS+NAA 0.2 mg/L+6-BA 3.0 mg/L was the best for regeneration culture.