CAJ | 학술논문

目的：研究两组同一分期预后显著不同的早期食管鳞状细胞癌（简称鳞癌）微小RNA （ miRNA）表达的差异，探索不同miRNA在早期食管鳞癌转移中的预后意义。方法采用TaqMan human microRNA 芯片技术分析预后显著不同的两组早期食管鳞癌381个miRNA表达情况。荧光定量PCR（ RT-PCR）方法验证差异miRNA表达结果。结果两组样本中共41个miRNA表达变化差异具有统计学意义（P＜0.05），数据经生物信息学分析、靶基因预测、筛选出靶基因显著性功能分析、信号通路分析，其中上调或下调7倍以上的miRNA为：miR-27a、miR-886-5p、miR-143。富集的靶基因为GRB2、SOS1、MAPK1、EGFR、CBL、SPRY2、RPS6KA5、IGF1R、NGFR、MAPK14、CREB1。这些基因与以下信号通路显著相关：Sprouty 调节的酪氨酸激酶信号通路、Erk1/Erk2丝裂原激活的蛋白激酶信号通路、转录因子CREB 和细胞外信号。结论 miR-27a、miR-886-5p、miR-143可能是早期食管鳞癌转移的潜在预后相关标志物，对早期食管鳞癌转移具有预测意义，这些标志物的检测对早期食管癌治疗方式选择具有指导意义。
목적：연구량조동일분기예후현저불동적조기식관린상세포암（간칭린암）미소RNA （ miRNA）표체적차이，탐색불동miRNA재조기식관린암전이중적예후의의。방법채용TaqMan human microRNA 심편기술분석예후현저불동적량조조기식관린암381개miRNA표체정황。형광정량PCR（ RT-PCR）방법험증차이miRNA표체결과。결과량조양본중공41개miRNA표체변화차이구유통계학의의（P＜0.05），수거경생물신식학분석、파기인예측、사선출파기인현저성공능분석、신호통로분석，기중상조혹하조7배이상적miRNA위：miR-27a、miR-886-5p、miR-143。부집적파기인위GRB2、SOS1、MAPK1、EGFR、CBL、SPRY2、RPS6KA5、IGF1R、NGFR、MAPK14、CREB1。저사기인여이하신호통로현저상관：Sprouty 조절적락안산격매신호통로、Erk1/Erk2사렬원격활적단백격매신호통로、전록인자CREB 화세포외신호。결론 miR-27a、miR-886-5p、miR-143가능시조기식관린암전이적잠재예후상관표지물，대조기식관린암전이구유예측의의，저사표지물적검측대조기식관암치료방식선택구유지도의의。
Objective To study the difference of microRNA ( miRNA ) expression between two groups of early stage ( pT1N0 ) esophageal squamous cell carcinoma ( ESCC ) patients who had different outcome and the prognostic significance of different miRNA in metastatic of early ESCC , and to identify useful prognostic markers in the selection of appropriate treatment for early ESCC patients.Methods TaqMan human miRNA arrays and bioinformatics were used to detect and analyze the expression profiles of miRNAs in the two groups , and RT-PCR was used to verify the differences in miRNA expression.Results The miRNA arrays revealed a total of 41 markedly changed miRNAs in the survival group compared with the death group.Bioinformatics analysis , prediction and significant function analyses of targeted genes and pathway analysis identified that miR-27a, miR-143 and miR-886-5p levels were increased or decreased by seven-folds or more.The enriched target genes were GRB2, SOS1, MAPK1, EGFR, CBL, SPRY2, RPS6KA5, IGF1R, NGFR, MAPK14 and CREB1.These genes were significantly related to the following signaling pathways , i.e.Sprouty regulation of tyrosine kinase signals pathway , Erk1/Erk2 Mapk signaling pathway and transcription factor CREB and its extracellular signals.Conclusions miR-27a, miR-886-5p, and miR-143 may be potential prognostic markers of metastasis for early ESCC.The detection of these miRNAs plays a directive role for the treatment options of early ESCC.The regulation of targeted genes and mechanism remain to be further studied.