CAJ | 학술논문

目的：探讨骨髓间充质干细胞抑制Ⅰ型糖尿病大鼠淋巴细胞增殖的免疫调节相关机制。方法：分离、培养并鉴定大鼠的骨髓间充质干细胞，采用MTT法观察其对Ⅰ型糖尿病大鼠淋巴细胞增殖能力的影响，采用流式细胞仪分析骨髓间充质干细胞对Ⅰ型糖尿病大鼠CD4＋CD25＋调节性T细胞比例、细胞周期和细胞凋亡的影响。结果：B组和C组的吸光度值明显低于A组，数据之间比较差异均有统计学意义（P＜0．05），C组吸光度值明显低于B组，数据之间比较差异有统计学意义（ P＜0．05）；B组和C组的CD4＋CD25＋调节性T细胞比例均明显高于A组，数据比较差异均有统计学意义（ P＜0．05）， C组CD4＋CD25＋调节性T细胞比例明显高于B组，数据比较差异有统计学意义（ P＜0．05）；B组和C组的细胞凋亡水平明显高于A组，数据比较差异有统计学意义（P＜0．05），C组细胞凋亡水平明显高于B组，数据比较差异有统计学意义（P＜0．05）。结论：骨髓间充质干细胞可以显著抑制Ⅰ型糖尿病大鼠淋巴细胞增殖，其机制可能通过调节CD4＋CD25＋调节性T细胞比例，促进细胞凋亡，继而影响T淋巴细胞的免疫功能，发挥其抑制能力。
목적：탐토골수간충질간세포억제Ⅰ형당뇨병대서림파세포증식적면역조절상관궤제。방법：분리、배양병감정대서적골수간충질간세포，채용MTT법관찰기대Ⅰ형당뇨병대서림파세포증식능력적영향，채용류식세포의분석골수간충질간세포대Ⅰ형당뇨병대서CD4＋CD25＋조절성T세포비례、세포주기화세포조망적영향。결과：B조화C조적흡광도치명현저우A조，수거지간비교차이균유통계학의의（P＜0．05），C조흡광도치명현저우B조，수거지간비교차이유통계학의의（ P＜0．05）；B조화C조적CD4＋CD25＋조절성T세포비례균명현고우A조，수거비교차이균유통계학의의（ P＜0．05）， C조CD4＋CD25＋조절성T세포비례명현고우B조，수거비교차이유통계학의의（ P＜0．05）；B조화C조적세포조망수평명현고우A조，수거비교차이유통계학의의（P＜0．05），C조세포조망수평명현고우B조，수거비교차이유통계학의의（P＜0．05）。결론：골수간충질간세포가이현저억제Ⅰ형당뇨병대서림파세포증식，기궤제가능통과조절CD4＋CD25＋조절성T세포비례，촉진세포조망，계이영향T림파세포적면역공능，발휘기억제능력。
Objective:To investigate the regulation mechanisms of the bone marrow mesenchymal stem cells on lymphocyte pro -liferation of type I diabetic rats .Methods:The rat bone marrow mesenchymal stem cells were isolated , cultured and identified and the effect on lymphocyte proliferation of type Ⅰdiabetic rat was observed by MTT assay , and analyze the CD 4 +CD25 +regulatory T cell ra-tio, cell cycle and apoptosis of type I diabetes rat by flow cytometric .Results:B and C groups was significantly lower than the absor-bance values of group A,the differences between the data were statistically significant (P<0.05), C group was significantly lower than group B absorbance values, the difference was significant (P<0.05);the CD4 +CD25 +regulatory T cells of B and C groups were sig-nificantly higher than group A, the differences of the data were statistically significant (P<0.05), the CD4 +CD25 +regulatory T cell ratio of C group significantly higher than that group B , the differences were statistically significant (P<0.05);the apoptosis levels of B and C groups were significantly higher than group A , the differences were statistically significant (P<0.05), the apoptosis levels of C group were significantly higher in group B , the differences were statistically significant (P<0.05).Conclusion:Bone marrow mesen-chymal stem cells can significantly inhibit lymphocyte proliferation of type Ⅰdiabetic rats, and it may regulate CD4 +CD25 +regulatory T cells, promote apoptosis, thereby affecting the immune function of T lymphocytes , and play its rejection.