中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
5期
673-676
,共4页
周发为%游文忠%覃仕锋%唐生尧
週髮為%遊文忠%覃仕鋒%唐生堯
주발위%유문충%담사봉%당생요
肾病综合征%激素敏感型%儿童%IL-4%IL-5%TGF-β%IL-10
腎病綜閤徵%激素敏感型%兒童%IL-4%IL-5%TGF-β%IL-10
신병종합정%격소민감형%인동%IL-4%IL-5%TGF-β%IL-10
Nephrotic syndrome%Steroid-sensitive%Children%IL-4%IL-5%TGF-β%IL-10
目的:探讨小儿激素敏感型肾病综合征( SSNS)治疗前与恢复期Th2细胞相关细胞因子IL-4、IL-5和Treg T细胞相关细胞因子TGF-β、IL-10水平的变化。方法:纳入36例SSNS患儿在治疗前和缓解期以及匹配的18例健康体检儿童,采用ELISA方法检测其血清IL-4、IL-5、TGF-β和IL-10水平,分析其水平变化与各临床指标的相关性。结果:SSNS患儿治疗前血清IL-4、IL-5显著高于缓解期以及健康对照组(P<0.01),肾病治疗前的血清TGF-β显著低于缓解期以及健康对照组(P<0.01),而各组之间的血清IL-10水平差异无统计学意义(P>0.05);SSNS治疗前的血清IL-4水平与血清白蛋白水平负相关(R2=-0.694,P=0.000);与血清胆固醇呈正相关(R2=0.658,P=0.000);血清TGF-β表达水平与血白蛋白呈正相关(R2=0.838,P=0.000),与血清胆固醇呈负相关(R2=-0.722,P=0.000)。结论:肾病综合征患儿血清IL-4、IL-5升高以及TGF-β水平降低与SSNS发病有关。
目的:探討小兒激素敏感型腎病綜閤徵( SSNS)治療前與恢複期Th2細胞相關細胞因子IL-4、IL-5和Treg T細胞相關細胞因子TGF-β、IL-10水平的變化。方法:納入36例SSNS患兒在治療前和緩解期以及匹配的18例健康體檢兒童,採用ELISA方法檢測其血清IL-4、IL-5、TGF-β和IL-10水平,分析其水平變化與各臨床指標的相關性。結果:SSNS患兒治療前血清IL-4、IL-5顯著高于緩解期以及健康對照組(P<0.01),腎病治療前的血清TGF-β顯著低于緩解期以及健康對照組(P<0.01),而各組之間的血清IL-10水平差異無統計學意義(P>0.05);SSNS治療前的血清IL-4水平與血清白蛋白水平負相關(R2=-0.694,P=0.000);與血清膽固醇呈正相關(R2=0.658,P=0.000);血清TGF-β錶達水平與血白蛋白呈正相關(R2=0.838,P=0.000),與血清膽固醇呈負相關(R2=-0.722,P=0.000)。結論:腎病綜閤徵患兒血清IL-4、IL-5升高以及TGF-β水平降低與SSNS髮病有關。
목적:탐토소인격소민감형신병종합정( SSNS)치료전여회복기Th2세포상관세포인자IL-4、IL-5화Treg T세포상관세포인자TGF-β、IL-10수평적변화。방법:납입36례SSNS환인재치료전화완해기이급필배적18례건강체검인동,채용ELISA방법검측기혈청IL-4、IL-5、TGF-β화IL-10수평,분석기수평변화여각림상지표적상관성。결과:SSNS환인치료전혈청IL-4、IL-5현저고우완해기이급건강대조조(P<0.01),신병치료전적혈청TGF-β현저저우완해기이급건강대조조(P<0.01),이각조지간적혈청IL-10수평차이무통계학의의(P>0.05);SSNS치료전적혈청IL-4수평여혈청백단백수평부상관(R2=-0.694,P=0.000);여혈청담고순정정상관(R2=0.658,P=0.000);혈청TGF-β표체수평여혈백단백정정상관(R2=0.838,P=0.000),여혈청담고순정부상관(R2=-0.722,P=0.000)。결론:신병종합정환인혈청IL-4、IL-5승고이급TGF-β수평강저여SSNS발병유관。
Objective:To clarify the dynamics of Th2 related cytokines IL-4、IL-5 and the Treg T cell related cytokines TGF-β、IL-10 in children with primary steroid-sensitive nephrotic syndrome ( SSNS) from the nephrotic phase before steroid treatment to the re-mission phase.Methods:All 36 SSNS patients and 18 healthy controls matched for age , gender, body weight and height were enrolled , and their clinical characteristics were evaluated .Serum cytokine level was measured by ELISA assay .The serum levels of interleukin (IL)-4, IL-5, IL-10 and transforming growth factor (TGF)-βwere compared and correlated with serum albumin and cholesterol .Re-sults:Serum IL-4 and IL-5 levels in SSNS of nephrotic phase were higher than serum IL-4 and IL-5 levels of SSNS of remission phase and the healthy control , respectively .The serum TGF-βlevels of the nephrotic phase were significantly lower than those of remission phase or control group , whereas the serum IL-10 levels showed no significant difference between nephrotic phase and remission phase of SSNS or control group.The serum IL-4 levels had a negative correlation with serum albumin (R2 =-0.694,P=0.000), and a posi-tive correlation with serum albumin levels (R2 =0.658,P=0.000), whereas the serum TGF-βlevels had a positive correlation with serum albumin (R2 =0.838,P=0.000), and a negative correlation with serum albumin levels , had (R2 =-0.722,P=0.000). Conclusion:This study indicates that IL-4, IL-5 and TGF-βlevel is related to the pathogenesis of pediatric SSNS .