CAJ | 학술논문

目的：探讨小干扰RNA长期作用HBV转基因小鼠对抑制乙肝病毒复制的评价。方法：siRNA表达载体经尾静脉注射转染HBV转基因小鼠，设立特异性siRNA组（pSilencer5．1/C2、pSilencer4．1/C2、pSilencer3．1/C2）、PBS对照组和阴性质粒对照组（ n＝10）。在注射后第6天、21天、1个月、3个月、6个月和9个月的不同时间经其内眦静脉采血，采用化学发光法定量检测小鼠血清中HBsAg水平，实时荧光定量PCR法检测HBV-DNA水平。结果：siRNA长期作用机体可使转基因小鼠血清HBsAg 和HBV-DNA水平显著降低，特异性siRNA组与PBS对照组相比，差异有显著统计学意义（P＜0．05）。而阴性质粒对照组与PBS组比较差异无统计学意义（ P＞0．05）。结论：基于载体的特异性siRNA长期作用HBV转基因小鼠可抑制HBV的复制和表达，该抑制作用是特异性的。
목적：탐토소간우RNA장기작용HBV전기인소서대억제을간병독복제적평개。방법：siRNA표체재체경미정맥주사전염HBV전기인소서，설립특이성siRNA조（pSilencer5．1/C2、pSilencer4．1/C2、pSilencer3．1/C2）、PBS대조조화음성질립대조조（ n＝10）。재주사후제6천、21천、1개월、3개월、6개월화9개월적불동시간경기내자정맥채혈，채용화학발광법정량검측소서혈청중HBsAg수평，실시형광정량PCR법검측HBV-DNA수평。결과：siRNA장기작용궤체가사전기인소서혈청HBsAg 화HBV-DNA수평현저강저，특이성siRNA조여PBS대조조상비，차이유현저통계학의의（P＜0．05）。이음성질립대조조여PBS조비교차이무통계학의의（ P＞0．05）。결론：기우재체적특이성siRNA장기작용HBV전기인소서가억제HBV적복제화표체，해억제작용시특이성적。
Objective:To investigation of the long-term-siRNA treatment with HBV transgene mice on inhibit replication of hepatitis B virus .Methods:The constructed siRNA expressed vectors was transfected HBV transgene mice by hydrodynamics -based in-jection via vena caudalis .Different groups were set including:specificity siRNA groups ( pSilencer5.1/C2,pSilencer4.1/C2,pSilenc-er3.1/C2),PBS group and negative vector group (n=10).The effect was observed in different periods (6 d,21 d,1 months,3 months, 6 months and 9 months after injection ) .HBsAg was analyzed by Chemiluminescence method , HBV-DNA was analyzed by real time quantitative PCR ( RQ-PCR) .Results:Compared with the PBS group , specificity siRNA groups showed decreased levels of HBsAg and HBV-DNA (P<0.05).Negative vector group did not show such changes ,there were no significant differences (P>0.05).Conclu-sion:The siRNA based on the expression vector can suppress the expression and replication of HBV in HBV transgene mice .The inhi-bition effects of long-term-siRNA treatment was specific .