CAJ | 학술논문

目的：筛选7型腺病毒的结合多肽，并研究其与腺病毒受体的相关性。方法：利用噬菌体肽库，筛选7型腺病毒的结合多肽，并制备其抗体，利用抗体进行免疫荧光实验，观察抗体与细胞膜的结合情况。结果：筛选到了GTS09多肽，其与噬菌体的复合物能特异的结合7型腺病毒，亲和常数可以达到1．93×1010 L/mol；制备了GTS09的兔多克隆抗体，免疫荧光显示，该抗体可以特异地结合在293细胞的细胞膜上。结论：抗GTS09的抗体可以特异的结合于细胞膜上，表明7型腺病毒受体可能与GTS09具有一定的同源性，为鉴定和分离7型腺病毒受体打下了基础。
목적：사선7형선병독적결합다태，병연구기여선병독수체적상관성。방법：이용서균체태고，사선7형선병독적결합다태，병제비기항체，이용항체진행면역형광실험，관찰항체여세포막적결합정황。결과：사선도료GTS09다태，기여서균체적복합물능특이적결합7형선병독，친화상수가이체도1．93×1010 L/mol；제비료GTS09적토다극륭항체，면역형광현시，해항체가이특이지결합재293세포적세포막상。결론：항GTS09적항체가이특이적결합우세포막상，표명7형선병독수체가능여GTS09구유일정적동원성，위감정화분리7형선병독수체타하료기출。
Objective:To screen the binding peptide against adenovirus type 7(Ad7) and evaluate the relevance with the ade-novirus receptor .Methods:Binding peptide against Ad 7 was screened by panning the phage display 12 peptides library .The antibody against the selected peptide was prepared and was used to study the binding to the membrane by immunofluorescence technique .Re-sults:Using Ad7 as the target protein , GTS09 peptide was selected from the phage display 12 peptides library by biopanning .GTS09-phage complex could significantly bind Ad 7, with the affinity constant up to 1.93 ×1010 L/mol;at the same time, immunofluorescence showed that antibody of GTS09 could specifically bind to membrane of 293 cell.Conclusion: Antibody against GTS09 peptide could specifically bind to membrane of 293 cell,which shows that the peptide may presumably have homology with the cell receptors of Ad 7.