亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2014年
1期
84-85
,共2页
翅梗石斛%组织培养%快速技术%B5培养基
翅梗石斛%組織培養%快速技術%B5培養基
시경석곡%조직배양%쾌속기술%B5배양기
Dendrobium trigonopus%tissue culture%rapid propagation%B 5 culture medium
以云南野生翅梗石斛Dendrobium trigonopus种子无菌萌发的试管苗为材料,初步探讨翅梗石斛的组织培养和快速繁殖技术。结果表明:B 5+NAA 0.5 mg·L-1+10%马铃薯汁和B 5+NAA 0.5 mg·L-1+6-BA 0.5 mg·L-1+10%马铃薯汁培养基分别对翅梗石斛种子的萌发与原球茎的增殖效果明显;B 5+NAA 0.5 mg·L-1+10%马铃薯汁+云南松树皮粉末2 g·L-1培养基对翅梗石斛的生根及壮苗效果较好。成苗后进行简单的药剂灭菌,便可直接移栽至锯末与刨花或锯末与树皮比例为1∶1的基质中,25 d后,成活率达95%以上。
以雲南野生翅梗石斛Dendrobium trigonopus種子無菌萌髮的試管苗為材料,初步探討翅梗石斛的組織培養和快速繁殖技術。結果錶明:B 5+NAA 0.5 mg·L-1+10%馬鈴藷汁和B 5+NAA 0.5 mg·L-1+6-BA 0.5 mg·L-1+10%馬鈴藷汁培養基分彆對翅梗石斛種子的萌髮與原毬莖的增殖效果明顯;B 5+NAA 0.5 mg·L-1+10%馬鈴藷汁+雲南鬆樹皮粉末2 g·L-1培養基對翅梗石斛的生根及壯苗效果較好。成苗後進行簡單的藥劑滅菌,便可直接移栽至鋸末與鑤花或鋸末與樹皮比例為1∶1的基質中,25 d後,成活率達95%以上。
이운남야생시경석곡Dendrobium trigonopus충자무균맹발적시관묘위재료,초보탐토시경석곡적조직배양화쾌속번식기술。결과표명:B 5+NAA 0.5 mg·L-1+10%마령서즙화B 5+NAA 0.5 mg·L-1+6-BA 0.5 mg·L-1+10%마령서즙배양기분별대시경석곡충자적맹발여원구경적증식효과명현;B 5+NAA 0.5 mg·L-1+10%마령서즙+운남송수피분말2 g·L-1배양기대시경석곡적생근급장묘효과교호。성묘후진행간단적약제멸균,편가직접이재지거말여포화혹거말여수피비례위1∶1적기질중,25 d후,성활솔체95%이상。
Yunnan wild Dendrobium trigonopus seed plantlets in vitro aseptic germination was used as test material to explore the tissue culture and rapid propagation techniques of D. trigonopus. The results showed that the culture medium of B5 +NAA 0.5 mg·L-1+10%potato juice and B5 +NAA 0.5 mg·L-1+6-BA 0.5 mg·L-1+10%potato juice had a significant effect to seedlings germination and protocorm proliferation of D. trigonopus respectively;the best rooting medium was B 5 + NAA 0.5 mg·L-1 + 10% potato juice + bark powder 2 g·L-1. Seedlings could be directly transplanted in the matrix of sawdust and wood shaving or sawdust and bark each half when they were simply sterilized, the survival rate exceeded 95%after transplanted 25 days.