亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2014年
1期
19-23
,共5页
曹雯静%李虹侠%唐源江%吴坤林
曹雯靜%李虹俠%唐源江%吳坤林
조문정%리홍협%당원강%오곤림
火焰兰属%染色体%核型%杂交组合%万代兰属
火燄蘭屬%染色體%覈型%雜交組閤%萬代蘭屬
화염란속%염색체%핵형%잡교조합%만대란속
Renanthera%chromosome%karyotype%hybridized combination%Vanda
采用染色体常规压片法对兰科Orchidaceae火焰兰属Renanthera内3个及与万代兰属Vanda间1个共4个杂交新组合F 1代核型进行研究。结果表明:4个杂交新组合F 1代中,麒麟火焰兰R. coccinea‘Qi Lin’、麒麟火焰兰×中华火焰兰R. sinica、(云南火焰兰R. imschootiana×中华火焰兰)×麒麟火焰兰、云南火焰兰×小花万代兰Vanda coerulescens的染色体数目依次为37、57、54、38;核型公式分别为:2n=2x+1=37=22m (2sat)+15sm (4sat)、2n=3x=57=39m (3sat)+12sm (3sat)+3st+3T、2n=3x=54=9M+27m+12sm+3st+3T、2n=2x=38=6M+14m+16sm+2T。核型不对称系数依次为:48.76、60.50、51.60、65.87。按Stebbins分类,均为“2B”型。4个新组合中,染色体存在非整倍体(三体)及多倍体(3×)现象,核形态显示了杂交新组合F 1代较丰富的变异特点。
採用染色體常規壓片法對蘭科Orchidaceae火燄蘭屬Renanthera內3箇及與萬代蘭屬Vanda間1箇共4箇雜交新組閤F 1代覈型進行研究。結果錶明:4箇雜交新組閤F 1代中,麒麟火燄蘭R. coccinea‘Qi Lin’、麒麟火燄蘭×中華火燄蘭R. sinica、(雲南火燄蘭R. imschootiana×中華火燄蘭)×麒麟火燄蘭、雲南火燄蘭×小花萬代蘭Vanda coerulescens的染色體數目依次為37、57、54、38;覈型公式分彆為:2n=2x+1=37=22m (2sat)+15sm (4sat)、2n=3x=57=39m (3sat)+12sm (3sat)+3st+3T、2n=3x=54=9M+27m+12sm+3st+3T、2n=2x=38=6M+14m+16sm+2T。覈型不對稱繫數依次為:48.76、60.50、51.60、65.87。按Stebbins分類,均為“2B”型。4箇新組閤中,染色體存在非整倍體(三體)及多倍體(3×)現象,覈形態顯示瞭雜交新組閤F 1代較豐富的變異特點。
채용염색체상규압편법대란과Orchidaceae화염란속Renanthera내3개급여만대란속Vanda간1개공4개잡교신조합F 1대핵형진행연구。결과표명:4개잡교신조합F 1대중,기린화염란R. coccinea‘Qi Lin’、기린화염란×중화화염란R. sinica、(운남화염란R. imschootiana×중화화염란)×기린화염란、운남화염란×소화만대란Vanda coerulescens적염색체수목의차위37、57、54、38;핵형공식분별위:2n=2x+1=37=22m (2sat)+15sm (4sat)、2n=3x=57=39m (3sat)+12sm (3sat)+3st+3T、2n=3x=54=9M+27m+12sm+3st+3T、2n=2x=38=6M+14m+16sm+2T。핵형불대칭계수의차위:48.76、60.50、51.60、65.87。안Stebbins분류,균위“2B”형。4개신조합중,염색체존재비정배체(삼체)급다배체(3×)현상,핵형태현시료잡교신조합F 1대교봉부적변이특점。
The chromosome number and karyotype of four hybridized combinations F1 generation including three intragenus combinations of Renanthera and one intergenus combination of Renanthera with Vanda were studied with normal squashing method in this paper. The results showed that the chromosome number of R. coccinea ‘Qi Lin’, R. coccinea ‘Qi Lin’ ′ R. sinica, (R. imschootiana′R. sinica)′R. coccinea‘Qi Lin’ and R. imschootiana′Vanda coerulescens was 37, 57, 54, 38, and the karyotypes of these were formulated as 2n=2x+1=37=22m(2sat)+15sm(4sat), 2n=3x=57=39m(3sat)+12sm(3sat)+3st+3T, 2n=3x=54=9M+27m+12sm+3st+3T, 2n=2x=38=6M+14m+16sm+2T in turn. Asysmmetry coefficient of nuclear is 48.76, 60.50, 51.60, 65.87 respectively. The karyotypes of all examined belonged to 2B type of Stebbins. Aneuploid and polyploid were found in these hybridized combinations. The karyotypes of four F 1 generations were richer in variation.