CAJ | 학술논문

目的：了解近50年中国不同地区分离的O1群El Tor型霍乱弧菌产毒株的生物表型特征变化。方法采用多粘菌素B敏感试验、第Ⅳ组霍乱噬菌体裂解试验、VP试验和溶血实验进行表型特征分析。结果生物型表型特征分析表明133株菌具有典型的El Tor生物型表型特征，其余251株菌呈现不典型的El Tor生物型表型特征；综合ctxB、rstR基因型和生物型表型特征分析，385株检测菌株中64株为典型El Tor生物型菌株，21株菌有典型的El Tor生物型表型特征但携带古典型ctxB基因，杂合型特征菌有280株，根据ctxB、rstR和表型特征的不同组合可再分为45个杂合型。结论中国O1群El Tor霍乱弧菌菌株呈现明显的表型多态性，传统的表型分型特征不能有效区分古典生物型和El Tor生物型菌株。
목적：료해근50년중국불동지구분리적O1군El Tor형곽란호균산독주적생물표형특정변화。방법채용다점균소B민감시험、제Ⅳ조곽란서균체렬해시험、VP시험화용혈실험진행표형특정분석。결과생물형표형특정분석표명133주균구유전형적El Tor생물형표형특정，기여251주균정현불전형적El Tor생물형표형특정；종합ctxB、rstR기인형화생물형표형특정분석，385주검측균주중64주위전형El Tor생물형균주，21주균유전형적El Tor생물형표형특정단휴대고전형ctxB기인，잡합형특정균유280주，근거ctxB、rstR화표형특정적불동조합가재분위45개잡합형。결론중국O1군El Tor곽란호균균주정현명현적표형다태성，전통적표형분형특정불능유효구분고전생물형화El Tor생물형균주。
Objective To understand the phenotypic diversity of toxigenic Vibrio cholerae O1 El Tor strains isolated from different provinces in China during the last 50 years. Methods Traditional biotyping testings including susceptibility to polymyxin B,sensitivity to groupⅣphage, Voges-Proskauer test and haemolysis of sheep erythrocytes were conducted. Results Data from Biotype-specific phenotype analysis revealed that only 133 isolates carryed the typical El Tor phenotypes while the other 251 isolates displayed atypical El Tor phenotypes. Combined with ctxB, rstR genotypes and phenotypic characteristics,64 isolates were identified as typical El Tor biotype,21 were El Tor variants that showing the typical El Tor biotype-specific phenotype but with ctxBclas . 280 isolates were defined as the hybrid groups with traits of both classical and El Tor biotypes that could be further classified into 45 groups,based on the combination of genotypes of ctxB,rstR and phenotypic characteristics. Conclusion Toxigenic Vibrio cholerae O1 El Tor strains that isolated from different provinces in China displayed high phenotypic diversity. The traditional biotype traits could not be used to correctly distinguish the two different biotypes.