CAJ | 학술논문

云南省德宏州2007年和2010年蚊虫及蚊媒病毒调查
운남성덕굉주2007년화2010년문충급문매병독조사
Investigation on mosquitoes and mosquito-borne viruses in Dehong prefecture,Yunnan province,2007 and 2010

万方数据

中华流行病学杂志中華流行病學雜誌 중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年 5期 528-532 ,共5页

冯云%张海林%付士红%杨卫红%章域震%王丕玉%杨捷%刘永华%董朝良%李轼%张保森%尹正留%董兴齐%王环宇%梁国栋

풍운%장해림%부사홍%양위홍%장역진%왕비옥%양첩%류영화%동조량%리식%장보삼%윤정류%동흥제%왕배우%량국동

蚊虫%流行性乙型脑炎病毒%盖塔病毒%淡色库蚊浓核病毒%分子生物学特征蚊蟲%流行性乙型腦炎病毒%蓋塔病毒%淡色庫蚊濃覈病毒%分子生物學特徵
문충%류행성을형뇌염병독%개탑병독%담색고문농핵병독%분자생물학특정
Mosquito%Japanese encephalitis virus%Getah virus%Culex pipiens pallens densovirus%Molecular biological characteristics

目的：调查云南省德宏州蚊虫和蚊媒病毒分布特点。方法2007年和2010年的7月在德宏州5个县市利用诱蚊灯采集蚊虫标本，采用C6/36和BHK-21细胞分离病毒，RT-PCR和序列分析方法鉴定病毒分离物。结果共采集到6属29种43634只蚊虫，其中三带喙库蚊构成比高达78.69%，中华按蚊构成比为14.77%，其他蚊种数量较少。从蚊虫中分离到6株病毒，经RT-PCR和进化分析表明，其中3株为基因Ⅰ型流行性乙型脑炎病毒（JEV），均分离自三带喙库蚊；1株盖塔病毒（GETV）分离自三带喙库蚊；2株淡色库蚊浓核病毒（CppDNV）分别分离自三带喙库蚊和迷糊按蚊。结论三带喙库蚊是德宏州优势蚊种和蚊媒病毒主要传播媒介，当地存在基因Ⅰ型JEV、GETV和CppDNV的流行，并与以往云南省及我国其他地区的分离株有较近亲缘关系。
목적：조사운남성덕굉주문충화문매병독분포특점。방법2007년화2010년적7월재덕굉주5개현시이용유문등채집문충표본，채용C6/36화BHK-21세포분리병독，RT-PCR화서렬분석방법감정병독분리물。결과공채집도6속29충43634지문충，기중삼대훼고문구성비고체78.69%，중화안문구성비위14.77%，기타문충수량교소。종문충중분리도6주병독，경RT-PCR화진화분석표명，기중3주위기인Ⅰ형류행성을형뇌염병독（JEV），균분리자삼대훼고문；1주개탑병독（GETV）분리자삼대훼고문；2주담색고문농핵병독（CppDNV）분별분리자삼대훼고문화미호안문。결론삼대훼고문시덕굉주우세문충화문매병독주요전파매개，당지존재기인Ⅰ형JEV、GETV화CppDNV적류행，병여이왕운남성급아국기타지구적분리주유교근친연관계。
Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Dehong prefecture,Yunnan province,China. Methods Mosquito samples were collected using the mosquito traps from five counties of Dehong prefecture on July,2007 and 2010. Mosquitoes were cell cultured for viral isolation,and positive isolates were identified using RT-PCR and sequence analysis. Results A total of 43 634 mosquitoes comprised of 29 species representing six genera were collected. Culex tritaeriorhynchus and Anopheles sinensis comprised 78.69% and 14.77% of the total. Six strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed three strains from Cx. tritaeriorhynchus,identified as genotypeⅠJapanese encephalitis virus(JEV). One strain was identified from Cx. tritaeriorhynchus,as Getah virus (GETV). Two strains isolated from Cx. tritaeriorhynchus and Anopheles vagus were identified as Culex pipiens pallens Densovirus(CppDNV). Conclusion Cx. tritaeriorhynchus had been the major species of mosquitoes and mainly transmitting vector of mosquito-borne viruses in Dehong prefecture. GenotypeⅠJEV,GETV and CppDNV were the vectors causing transmission of mosquitoe-borne diseases in this area. Data from phylogenetic analysis showed that these newly discovered isolates seemed to have had close relationship with those viruses previously circulating in Yunnan and other provinces of China.