CAJ | 학술논문

目的：了解山东省烟台市人和动物发热伴血小板减少综合征布尼亚病毒（SFTSV）感染及流行情况。方法2011年4-11月分别在烟台市的蓬莱和莱州2个SFTS病例高发地区连续采集与人密切接触的5种家养动物（羊、牛、猪、犬、鸡）血清标本3576份，应用双抗原夹心ELISA方法和Real time RT-PCR方法检测血清中SFTSV总抗体和病毒核酸；观察不同月份感染情况；采集两地人群血清2590份，应用间接ELISA方法检测SFTSV IgG抗体；用Vero细胞从核酸阳性的人和动物血清中分离病毒，通过RT-PCR方法对SFTSV S片段进行序列扩增、同源性分析。结果3576份动物血清标本中SFTSV血清总抗体阳性1439份，阳性率为40.24%，病毒核酸阳性163份，阳性率为4.56%。其中羊、牛、鸡、犬、猪抗体阳性率分别为62.78%、52.97%、45.56%、28.73%和1.45%，核酸阳性率分别为5.72%、4.63%、3.02%、5.25%和3.73%。动物体内的抗原抗体随季节消长而变化。2590份人群血清SFTSV IgG抗体阳性率为5.41%。对10株来自人的毒株和3株来自动物的毒株进行S片段基因序列扩增分析，显示其同源性在95.23%～100.00%，与国内其他省市分离毒株比较，其同源性在94.72%～99.13%，高度同源。结论烟台地区存在SFTSV流行，人与家养动物普遍易感，其基因序列高度同源，提示家养动物可能作为SFTSV的增殖宿主和扩散宿主，应引起高度重视。
목적：료해산동성연태시인화동물발열반혈소판감소종합정포니아병독（SFTSV）감염급류행정황。방법2011년4-11월분별재연태시적봉래화래주2개SFTS병례고발지구련속채집여인밀절접촉적5충가양동물（양、우、저、견、계）혈청표본3576빈，응용쌍항원협심ELISA방법화Real time RT-PCR방법검측혈청중SFTSV총항체화병독핵산；관찰불동월빈감염정황；채집량지인군혈청2590빈，응용간접ELISA방법검측SFTSV IgG항체；용Vero세포종핵산양성적인화동물혈청중분리병독，통과RT-PCR방법대SFTSV S편단진행서렬확증、동원성분석。결과3576빈동물혈청표본중SFTSV혈청총항체양성1439빈，양성솔위40.24%，병독핵산양성163빈，양성솔위4.56%。기중양、우、계、견、저항체양성솔분별위62.78%、52.97%、45.56%、28.73%화1.45%，핵산양성솔분별위5.72%、4.63%、3.02%、5.25%화3.73%。동물체내적항원항체수계절소장이변화。2590빈인군혈청SFTSV IgG항체양성솔위5.41%。대10주래자인적독주화3주래자동물적독주진행S편단기인서렬확증분석，현시기동원성재95.23%～100.00%，여국내기타성시분리독주비교，기동원성재94.72%～99.13%，고도동원。결론연태지구존재SFTSV류행，인여가양동물보편역감，기기인서렬고도동원，제시가양동물가능작위SFTSV적증식숙주화확산숙주，응인기고도중시。
Objective To learn the prevalence of infection of human and animals severe fever with thrombocytopenia syndrome bunyavirus(SFTSV)in Yantai,Shandong province,and to analyze the pathogenic features of SFTSV as well as its relationship between human and animal hosts. Methods From April to November in 2011,3 576 serum samples were collected from domesticated animals,including sheep,cattle,pigs,dogs,chickens,in Laizhou and Penglai areas where fever with thrombocytopenia syndrome frequently occured among local residents. Total SFTSV antibodies and virus-specific nucleic acids of the serum were tested by ELISA and Real time RT-PCR,respectively. SFTSV infection on each animal was observed in different months. 2 590 human serum samples were also collected in Laizhou and Penglai areas,with IgG antibodies tested by ELISA. Virus was isolated with Vero cells from the serum which SFTSV viral nucleic acids were positive. S fragments were amplified by RT-PCR and sequenced,with homology analysis conducted on these sequences. Results The overall positive rate of serum samples from animals on the total SFTSV antibodies was 40.24%(1 439/3 576)while the positive rate for specific nucleic acids was 4.56%(163/3 576). The positive rates for SFTSV antibodies were 62.78%,52.97%,45.56%,28.73%,1.45%and the positive rates for specific nucleic acids were 5.72%,4.63%,3.02%,5.25%and 3.73%,in sheep,cattle,chickens,dogs, pigs,respectively. The antigens/antibodies for SFTSV in animals changed seasonally. The overall positive rate for SFTSV IgG antibody from 2 590 human samples was 5.41%. Thirteen virus strains were isolated from these serum samples(10 strains from human and 3 strains from animals). The nucleotide homology of 13S fragments’sequences ranged from 95.23%to 100.00%and the nucleotide homology with the isolates from other provinces were between 94.72%and 99.13%. The homology was considered to be high. Conclusion High prevalence of SFTSV infections occured both in human and domestic animals in Yantai city. The nucleotide sequences of SFTSV were highly homologous among human and domestic animals. The findings suggested that domesticated animals might serve as SFTSV proliferation and the hosts for transmission thus should be attached great importance.