中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
5期
514-518
,共5页
费满冬%李佳圆%杜旌畅%游嘉%张韶凯%何微%康乐妮%赵方辉%乔友林%司玉芝%樊小平%陈汶
費滿鼕%李佳圓%杜旌暢%遊嘉%張韶凱%何微%康樂妮%趙方輝%喬友林%司玉芝%樊小平%陳汶
비만동%리가원%두정창%유가%장소개%하미%강악니%조방휘%교우림%사옥지%번소평%진문
人乳头瘤病毒%基因型别%血清抗体%酶联免疫吸附试验
人乳頭瘤病毒%基因型彆%血清抗體%酶聯免疫吸附試驗
인유두류병독%기인형별%혈청항체%매련면역흡부시험
Human papillomavirus%Genotypes%Serum antibody%Enzyme-linked immunosorbent assay
目的：分析HPV16和18型（HPV16/18）血清抗体在高危人群中的分布。方法召回952名参加2011年河南省新密市子宫颈癌筛查阳性妇女，于2012年采集子宫颈口脱落细胞检测HPV DNA，采用ELISA检测血清HPV16/18抗体。结果952名妇女中230例（24.2%）HPV DNA阳性，HPV16/18病毒样颗粒（VLP）抗体阳性率分别为23.2%和6.5%，任意一种HPV16/18 VLP抗体阳性率为26.8%。HPV16/18抗体GMT分别为79.1 YU（Yangshengtang Unit）/ml和125.0 YU/ml。HPV16抗体阳性率在不同年龄、不同病毒载量和不同子宫颈病变组的差异有统计学意义（P＜0.05）；HPV18抗体阳性率与病毒载量相关（P＜0.01），且在不同病理等级中其滴度分布不同，差异有统计学意义（P＜0.01）。结合两年HPV DNA检测结果，HPV持续感染的妇女HPV16/18抗体阳性率高于HPV新发感染和无HPV感染妇女，差异有统计学意义（P＜0.001）；相对于无HPV感染妇女，HPV一过性感染者HPV16抗体阳性率和滴度均较高，差异有统计学意义（P＜0.001）。结论 HPV16/18抗体在调查的高危妇女中阳性率较高，且与年龄、病毒载量、子宫颈病变程度以及既往感染史有关，病毒载量高、病变程度重或有既往感染史者更易产生抗体。
目的：分析HPV16和18型（HPV16/18）血清抗體在高危人群中的分佈。方法召迴952名參加2011年河南省新密市子宮頸癌篩查暘性婦女，于2012年採集子宮頸口脫落細胞檢測HPV DNA，採用ELISA檢測血清HPV16/18抗體。結果952名婦女中230例（24.2%）HPV DNA暘性，HPV16/18病毒樣顆粒（VLP）抗體暘性率分彆為23.2%和6.5%，任意一種HPV16/18 VLP抗體暘性率為26.8%。HPV16/18抗體GMT分彆為79.1 YU（Yangshengtang Unit）/ml和125.0 YU/ml。HPV16抗體暘性率在不同年齡、不同病毒載量和不同子宮頸病變組的差異有統計學意義（P＜0.05）；HPV18抗體暘性率與病毒載量相關（P＜0.01），且在不同病理等級中其滴度分佈不同，差異有統計學意義（P＜0.01）。結閤兩年HPV DNA檢測結果，HPV持續感染的婦女HPV16/18抗體暘性率高于HPV新髮感染和無HPV感染婦女，差異有統計學意義（P＜0.001）；相對于無HPV感染婦女，HPV一過性感染者HPV16抗體暘性率和滴度均較高，差異有統計學意義（P＜0.001）。結論 HPV16/18抗體在調查的高危婦女中暘性率較高，且與年齡、病毒載量、子宮頸病變程度以及既往感染史有關，病毒載量高、病變程度重或有既往感染史者更易產生抗體。
목적：분석HPV16화18형（HPV16/18）혈청항체재고위인군중적분포。방법소회952명삼가2011년하남성신밀시자궁경암사사양성부녀，우2012년채집자궁경구탈락세포검측HPV DNA，채용ELISA검측혈청HPV16/18항체。결과952명부녀중230례（24.2%）HPV DNA양성，HPV16/18병독양과립（VLP）항체양성솔분별위23.2%화6.5%，임의일충HPV16/18 VLP항체양성솔위26.8%。HPV16/18항체GMT분별위79.1 YU（Yangshengtang Unit）/ml화125.0 YU/ml。HPV16항체양성솔재불동년령、불동병독재량화불동자궁경병변조적차이유통계학의의（P＜0.05）；HPV18항체양성솔여병독재량상관（P＜0.01），차재불동병리등급중기적도분포불동，차이유통계학의의（P＜0.01）。결합량년HPV DNA검측결과，HPV지속감염적부녀HPV16/18항체양성솔고우HPV신발감염화무HPV감염부녀，차이유통계학의의（P＜0.001）；상대우무HPV감염부녀，HPV일과성감염자HPV16항체양성솔화적도균교고，차이유통계학의의（P＜0.001）。결론 HPV16/18항체재조사적고위부녀중양성솔교고，차여년령、병독재량、자궁경병변정도이급기왕감염사유관，병독재량고、병변정도중혹유기왕감염사자경역산생항체。
Objective To explore the distribution of serum antibodies against human papillomavirus(HPV)16/18 among women at high-risk for cervical cancer. Methods All women when tested positive for anyone of the cervical cancer screening programs,from Xinmi county of Henan province in 2011,were recruited as the subjects of this study. Cervical exfoliated cells were collected,using cervical brush for HPV DNA testing,and 10 ml venous blood was drawn for HPV-16, 18 serum antibodies testing,by enzyme-linked immunosorbent assay. Results Among the 952 women unders study,230 cases(24.2%)showed HPV DNA positive,with positivity rates of HPV16 and 18 L1 virus-like particle(VLP)antibodies as 23.2%and 6.5%,respectively. The overall positivity rate of any type of HPV16,18 VLP antibodies was 26.8%. Geometric means of HPV16,18 VLP antibody titers were 79.1(Yangshengtang Unit,YU/ml)and 125.0(YU/ml). Positivity rate of HPV16 antibody was significantly associated with age,viral load of HPV DNA,and cervical lesion severity (P<0.05). Seropositvity of HPV18 was also increasing with the increase of viral load (P<0.01) with different cervical lesion significantly showing different titer of HPV18 antibody (P<0.01). Based on the results of HPV DNA detection among the two years of study,women with HPV persistent infection showed significant higher positive rate of HPV16/18 antibodies than women who did not have HPV infection or emerging infection (P<0.001). When comparing to those women without HPV infection,the ones with transient infection showed higher seropositivity rates on both HPV16 antibodies and titer of HPV16 antibody (P<0.001). Conclusion Seroprevalence rates on HPV16 and 18 among the unvaccinated high-risk women in Henan were high. Prevalence of both HPV16 and 18 antibodies were correlated with age,viral load,cervical lesion and history of infection. Women with high viral load,high grade cervical lesion or history of infection would more likely to be seropositive.
