中外食品工业(下半月)
中外食品工業(下半月)
중외식품공업(하반월)
Sino-foreign Food Industry
2014年
2期
15-20
,共6页
周鑫魁%洪霞%钱滢文%高志莹%吴永宝%刘琦
週鑫魁%洪霞%錢瀅文%高誌瑩%吳永寶%劉琦
주흠괴%홍하%전형문%고지형%오영보%류기
ELISA%莱克多巴胺%间接非竞争法%低检出限
ELISA%萊剋多巴胺%間接非競爭法%低檢齣限
ELISA%래극다파알%간접비경쟁법%저검출한
ractopamine%ELISA%Indirect non%competition law%Low detection limit
本快速测定方法使用甲醇提取液超声波提取动物源性制品中莱克多巴胺,采用样品与抗体预混的间接非竞争法测定样品中的莱克多巴胺。没有采用常规的间接竞争法,而是预先混合抗体和待测物质,使得检出限底,达到0.0044ng/mL,其含量在0.0044~11ng/mL浓度范围内呈良好线性关系,相关系数R2=0.9799,回收率在65%~89%之间,具有较好的稳定性、重现性和回收率,能满足实验室的检测要求。
本快速測定方法使用甲醇提取液超聲波提取動物源性製品中萊剋多巴胺,採用樣品與抗體預混的間接非競爭法測定樣品中的萊剋多巴胺。沒有採用常規的間接競爭法,而是預先混閤抗體和待測物質,使得檢齣限底,達到0.0044ng/mL,其含量在0.0044~11ng/mL濃度範圍內呈良好線性關繫,相關繫數R2=0.9799,迴收率在65%~89%之間,具有較好的穩定性、重現性和迴收率,能滿足實驗室的檢測要求。
본쾌속측정방법사용갑순제취액초성파제취동물원성제품중래극다파알,채용양품여항체예혼적간접비경쟁법측정양품중적래극다파알。몰유채용상규적간접경쟁법,이시예선혼합항체화대측물질,사득검출한저,체도0.0044ng/mL,기함량재0.0044~11ng/mL농도범위내정량호선성관계,상관계수R2=0.9799,회수솔재65%~89%지간,구유교호적은정성、중현성화회수솔,능만족실험실적검측요구。
This rapid determination method using methanol extract ultrasonic extraction ractopamine in products of animal origin, Using samples and antibody indirect non premixed competition law to determine the ractopamine in the samples.Instead of the conventional indirect competition law, premixed antibody and material under test, the detection limit, 0.0044 ng/mL, its content in 0.0044~11 ng/mL concentration had good linear relationship, within the scope of correlation coefficient R2=0.9799,recovery is between 65%~89%,Has a good stability, repeatability and recovery, can satisfy the requirement of the laboratory testing.