浙江大学学报(医学版)
浙江大學學報(醫學版)
절강대학학보(의학판)
JOURNAL OF ZHEJIANG UNIVERSITY MEDICAL SCIENCES
2014年
2期
175-179
,共5页
柏科/化学%酚类%抗氧化剂
柏科/化學%酚類%抗氧化劑
백과/화학%분류%항양화제
Cupressaceae/chemistry%Phenols%Antioxidants
目的:提高沙地柏多酚的纯化程度并考察其抗氧化活性。方法:采用Folin-Ciocalteu法测定沙地柏的多酚含量,经筛选用HPD-700大孔树脂纯化沙地柏多酚。通过对沙地柏多酚,1,1-二苯基-2-三硝基苯肼( DPPH)自由基、超氧阴离子、羟自由基的清除能力、总抗氧化能力及三价铁离子( Fe3+)还原能力的测定来评价其抗氧化能力。结果:经纯化后,沙地柏多酚纯度由0.53‰提高到9.95‰。抗氧化活性测定结果表明,沙地柏多酚对DPPH自由基的抑制率和Fe3+还原能力的维生素C当量质量浓度分别为9.4μg/ml、30.09μg/ml,对超氧阴离子和羟自由基的清除能力分别为151.83 U/ml、204.59 U/ml,总抗氧化能力为72.68 U/ml。结论:沙地柏多酚具有较强的抗氧化活性,值得进一步研究其药理作用。
目的:提高沙地柏多酚的純化程度併攷察其抗氧化活性。方法:採用Folin-Ciocalteu法測定沙地柏的多酚含量,經篩選用HPD-700大孔樹脂純化沙地柏多酚。通過對沙地柏多酚,1,1-二苯基-2-三硝基苯肼( DPPH)自由基、超氧陰離子、羥自由基的清除能力、總抗氧化能力及三價鐵離子( Fe3+)還原能力的測定來評價其抗氧化能力。結果:經純化後,沙地柏多酚純度由0.53‰提高到9.95‰。抗氧化活性測定結果錶明,沙地柏多酚對DPPH自由基的抑製率和Fe3+還原能力的維生素C噹量質量濃度分彆為9.4μg/ml、30.09μg/ml,對超氧陰離子和羥自由基的清除能力分彆為151.83 U/ml、204.59 U/ml,總抗氧化能力為72.68 U/ml。結論:沙地柏多酚具有較彊的抗氧化活性,值得進一步研究其藥理作用。
목적:제고사지백다분적순화정도병고찰기항양화활성。방법:채용Folin-Ciocalteu법측정사지백적다분함량,경사선용HPD-700대공수지순화사지백다분。통과대사지백다분,1,1-이분기-2-삼초기분정( DPPH)자유기、초양음리자、간자유기적청제능력、총항양화능력급삼개철리자( Fe3+)환원능력적측정래평개기항양화능력。결과:경순화후,사지백다분순도유0.53‰제고도9.95‰。항양화활성측정결과표명,사지백다분대DPPH자유기적억제솔화Fe3+환원능력적유생소C당량질량농도분별위9.4μg/ml、30.09μg/ml,대초양음리자화간자유기적청제능력분별위151.83 U/ml、204.59 U/ml,총항양화능력위72.68 U/ml。결론:사지백다분구유교강적항양화활성,치득진일보연구기약리작용。
Objective:To purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties .Methods: Polyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700 , and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry .The antioxidant properties of polyphenols were evaluated by total antioxidant capacity ( T-AOC ) and its activeties of scavenging DPPH (1,1diphenyl-2-picry-hydrazyl) radicals, superoxide anion ( O2 · -), hydroxyl free radicals (OH· ) and ferric reducing antioxidant power (FRAP).Results: After purification, the purity of polyphenols increased from 0.53‰ to 9.95‰.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml.Its scavenging capacity for superoxide anion ( O2 · -) and hydroxyl free radicals ( OH · ) was 151.83 U/ml and 204.59 U/ml.The total antioxidant capacity was 72.68 U/ml.Conclusion:Polyphenols from Sabina vulgaris Antoine have high antioxidant properties , suggesting that it worth further study of its pharmacological effects .