CAJ | 학술논문

目的：建立适用于体外细胞模型的OATP1 B1手性底物非索非那定柱前手性衍生化分析方法，为测定细胞中非索非那定对映体提供分析手段。方法：选择R-（＋）-苯乙基异氰酸酯作为手性衍生化试剂，与非索非那定生成氨基甲酸酯衍生物，应用液相色谱-串联质谱法确定对映体衍生物色谱峰，通过反相高效液相色谱法实现对映体的分离分析。结果：在建立的手性分离条件下，成功实现非索非那定对映体分离分析，非索非那定两个对映体衍生物在25～100 ng/ml浓度范围内线性关系良好（R2＝0．9992，0．9989），日内、日间精密度均小于10％。结论：本实验建立的非索非那定对映体柱前手性衍生化反相高效液相色谱分析方法灵敏、准确，可用于体外细胞模型中盐酸非索非那定立体选择性分析。
목적：건립괄용우체외세포모형적OATP1 B1수성저물비색비나정주전수성연생화분석방법，위측정세포중비색비나정대영체제공분석수단。방법：선택R-（＋）-분을기이청산지작위수성연생화시제，여비색비나정생성안기갑산지연생물，응용액상색보-천련질보법학정대영체연생물색보봉，통과반상고효액상색보법실현대영체적분리분석。결과：재건립적수성분리조건하，성공실현비색비나정대영체분리분석，비색비나정량개대영체연생물재25～100 ng/ml농도범위내선성관계량호（R2＝0．9992，0．9989），일내、일간정밀도균소우10％。결론：본실험건립적비색비나정대영체주전수성연생화반상고효액상색보분석방법령민、준학，가용우체외세포모형중염산비색비나정입체선택성분석。
Objective: To establish a precolumn chiral derivatization method for determination of fexofenadine enantiomers , a chiral substrate of OATP1B1, in cellular model.Methods: R-( +)-phenylethylisocyanate was selected as chiral derivatization reagent , which was reacted with fexofenadine to form carbamate derivatives . Enantiomers were identified by LC/MS and separated by RP-HPLC.Results:Under the experimental conditions , the fexofenadine enantiomers were separated completely .The standard curve was linear over the concentration range of 25-100 ng/ml (R2 =0.9992, 0.9989).Accuracy was 101.1%and 98.3%, intra-precision was 2.4%and 3.1%, inter-precision was 3.1% and 4.0% for D1 and D2, respectively.Conclusion: The method established is sensitive and accurate for determination of fexofenadine enantiomers in cells .