CAJ | 학술논문

目的：研究黄芩素与氟康唑合用对白念珠菌生物被膜形成的影响。方法采用激光共聚焦显微镜观察黄芩素与氟康唑合用对白念珠菌生物被膜生长形态的影响；采用 XTT 法考察黄芩素与氟康唑合用对白念珠菌生物被膜形成能力的影响；应用水-烃两相测定实验考察黄芩素与氟康唑合用对白念珠菌生物被膜细胞表面疏水性（ Cell surface hydrophobicity， CSH）的影响；应用实时定量 RT-PCR（Real Time RT-PCR）实验考察黄芩素与氟康唑合用对白念珠菌 CSH1、EFG1、HWP1、ALS1基因表达的影响。结果黄芩素与氟康唑合用能够协同抑制白念珠菌生物被膜的形成，经黄芩素与氟康唑处理的白念珠菌不能形成正常的生物被膜，其生长动力学及细胞表面疏水性下降，细胞疏水性相关基 CSH1、菌丝形成调控基因EFG1、黏附相关基因 HWP1基因的表达水平降低。结论黄芩素与氟康唑合用可协同抑制白念珠菌生物被膜的形成。
목적：연구황금소여불강서합용대백념주균생물피막형성적영향。방법채용격광공취초현미경관찰황금소여불강서합용대백념주균생물피막생장형태적영향；채용 XTT 법고찰황금소여불강서합용대백념주균생물피막형성능력적영향；응용수-경량상측정실험고찰황금소여불강서합용대백념주균생물피막세포표면소수성（ Cell surface hydrophobicity， CSH）적영향；응용실시정량 RT-PCR（Real Time RT-PCR）실험고찰황금소여불강서합용대백념주균 CSH1、EFG1、HWP1、ALS1기인표체적영향。결과황금소여불강서합용능구협동억제백념주균생물피막적형성，경황금소여불강서처리적백념주균불능형성정상적생물피막，기생장동역학급세포표면소수성하강，세포소수성상관기 CSH1、균사형성조공기인EFG1、점부상관기인 HWP1기인적표체수평강저。결론황금소여불강서합용가협동억제백념주균생물피막적형성。
Objective To investigate the effect of baicalein(BE)in combination with fluconazole(FLC)on Candida albicans biofilm formation. Methods The inhibition of C. albicans biofilm by BE in combination with FLC was determined by confocal scan-ning laser microscopy and XTT methods. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity (CSH). The expression of CSH1,EFG1,HWP1,ALS1 mRNA was measured by Real time RT-PCR. Results BE in combination with FLC could inhibit the formation of C. albicans biofilm. The cell surface hydrophobicity in the BE and FLC-treated group was lower than that in the groups treated by BE or FLC alone. Consistent with this,BE and FLC-treated biofilm cells expressed lower lev-els of CSH1,EFG1,HWP1 mRNA than the cells grown in the presence of BE or FLC alone. Conclusions BE in combination with FLC could inhibit the formation of C. albicans biofilm.