中国烟草学报
中國煙草學報
중국연초학보
ACTA TABACARIA SINICA
2014年
2期
99-103
,共5页
烟草%SOC1%转基因%光合%抗坏血酸
煙草%SOC1%轉基因%光閤%抗壞血痠
연초%SOC1%전기인%광합%항배혈산
tobacco%SOC1%transgene%photosynthetic%ascorbic acid
为明确SOC1基因在叶片中的生理作用，克隆到烟草中SOC1基因的完整开放阅读框，全长806 bp。农杆菌介导转化烟草后，获得26棵转基因植株，其中23棵鉴定为阳性植株，转化率为88.46%。与野生型烟草K326相比，获得的SOC1过表达烟株开花提前，株高增加，叶片宽大。半定量RT-PCR结果显示，SOC1过表达不影响叶片中Rubisco大亚基、蔗糖磷酸合成酶和蔗糖合成酶基因的表达水平，但提高了抗坏血酸氧化酶的表达水平；提高了碳酸酐酶活性、蔗糖含量和还原性抗坏血酸含量，并降低了抗坏血酸过氧化物酶活性。表明SOC1可能通过影响氧化还原状态而提高碳酸酐酶活性和光合速率。
為明確SOC1基因在葉片中的生理作用，剋隆到煙草中SOC1基因的完整開放閱讀框，全長806 bp。農桿菌介導轉化煙草後，穫得26棵轉基因植株，其中23棵鑒定為暘性植株，轉化率為88.46%。與野生型煙草K326相比，穫得的SOC1過錶達煙株開花提前，株高增加，葉片寬大。半定量RT-PCR結果顯示，SOC1過錶達不影響葉片中Rubisco大亞基、蔗糖燐痠閤成酶和蔗糖閤成酶基因的錶達水平，但提高瞭抗壞血痠氧化酶的錶達水平；提高瞭碳痠酐酶活性、蔗糖含量和還原性抗壞血痠含量，併降低瞭抗壞血痠過氧化物酶活性。錶明SOC1可能通過影響氧化還原狀態而提高碳痠酐酶活性和光閤速率。
위명학SOC1기인재협편중적생리작용，극륭도연초중SOC1기인적완정개방열독광，전장806 bp。농간균개도전화연초후，획득26과전기인식주，기중23과감정위양성식주，전화솔위88.46%。여야생형연초K326상비，획득적SOC1과표체연주개화제전，주고증가，협편관대。반정량RT-PCR결과현시，SOC1과표체불영향협편중Rubisco대아기、자당린산합성매화자당합성매기인적표체수평，단제고료항배혈산양화매적표체수평；제고료탄산항매활성、자당함량화환원성항배혈산함량，병강저료항배혈산과양화물매활성。표명SOC1가능통과영향양화환원상태이제고탄산항매활성화광합속솔。
Open reading frame of SUPPRESSOR OF OVEREXPRESSION OF CONSTANTS 1 (SOC1) gene in tobacco was cloned with full length of 806 bp to investigate its physiological function in tobacco. Agrobacterium-mediated leaf-disk transformations were used to create 26 SOC1 transgenic tobacco plants. Among which 23 were identified as positive plants with transformation rate of 88.46%. Compared with tobacco variety K326, SOC1 over-expression plants had early floral time, higher plant heights and wider and longer leaves. Semi-quantitative RT-PCR results showed that over-expression of SOC1 did not affect expression levels of Rubisco large subunit, sucrose phosphate synthetase and sucrose synthetase in leaves, but increased the level of ascorbare oxidase. It also increased carbonic anhydrase activity, concentrations of sucrose and ascorbic acid, and reduced ascorbate peroxidase activities. These results suggested that SOC1 increase carbonic anhydrase activity and photosynthesis by changing ascorbate redox state.
