江汉大学学报（自然科学版）
江漢大學學報（自然科學版）
강한대학학보（자연과학판）
JOURNAL OF JIANGHAN UNIVERSITY（NATURAL SCIENCES）
2014年
2期
83-87
,共5页
李长雷%马宝苗%柳威%陈晓青%易卉玲%舒细记
李長雷%馬寶苗%柳威%陳曉青%易卉玲%舒細記
리장뢰%마보묘%류위%진효청%역훼령%서세기
体细胞%克隆西农莎能奶山羊%微卫星DNA分析%电泳%银染
體細胞%剋隆西農莎能奶山羊%微衛星DNA分析%電泳%銀染
체세포%극륭서농사능내산양%미위성DNA분석%전영%은염
somatic cell%cloned Xinong Saanen dairy goat%microsatellite DNA analysis%electro-phoresis%silver staining
目的：利用微卫星DNA 技术对通过体细胞克隆技术获得的两只西农莎能奶山羊进行鉴定。方法提取两只克隆西农莎能奶山羊、两只受体西农莎能奶山羊母羊、西农莎能奶山羊供体细胞和两只对照组西农莎能奶山羊母羊的基因组DNA，通过5对具有显著多态性差异的引物扩增微卫星DNA序列，并对其进行微卫星DNA分析。结果两只克隆西农莎能奶山羊和供体细胞的基因型完全一致，受体母羊和对照组母羊的微卫星DNA多态性则与前两者均不相同。结论两只克隆西农莎能奶山羊基因组DNA来源于供体细胞。
目的：利用微衛星DNA 技術對通過體細胞剋隆技術穫得的兩隻西農莎能奶山羊進行鑒定。方法提取兩隻剋隆西農莎能奶山羊、兩隻受體西農莎能奶山羊母羊、西農莎能奶山羊供體細胞和兩隻對照組西農莎能奶山羊母羊的基因組DNA，通過5對具有顯著多態性差異的引物擴增微衛星DNA序列，併對其進行微衛星DNA分析。結果兩隻剋隆西農莎能奶山羊和供體細胞的基因型完全一緻，受體母羊和對照組母羊的微衛星DNA多態性則與前兩者均不相同。結論兩隻剋隆西農莎能奶山羊基因組DNA來源于供體細胞。
목적：이용미위성DNA 기술대통과체세포극륭기술획득적량지서농사능내산양진행감정。방법제취량지극륭서농사능내산양、량지수체서농사능내산양모양、서농사능내산양공체세포화량지대조조서농사능내산양모양적기인조DNA，통과5대구유현저다태성차이적인물확증미위성DNA서렬，병대기진행미위성DNA분석。결과량지극륭서농사능내산양화공체세포적기인형완전일치，수체모양화대조조모양적미위성DNA다태성칙여전량자균불상동。결론량지극륭서농사능내산양기인조DNA래원우공체세포。
Objective To identify two Xinong Saanen dairy goats whom were obtained through so-matic cell cloning technology with microsatellite DNA technology. Methods Extracting genomic DNA of two cloned Xinong Saanen dairy goats,two recipient Xinong Saanen dairy goats,donor Xi-nong Saanen dairy goat and two control group Xinong Saanen goats,then microsatellite DNA analy-sis was performed with microsatellite DNA sequences which were amplified from five pairs signifi-cant different polymorphic primer. Results Genotype of two Xinong Saanen dairy goats are the same as the donor cells,but the microsatellite DNA polymorphism of recipient goat and control group goat are different from the above mentioned. Conclusion Genomic DNA of two Xinong Saa-nen dairy goats come from the donor cells.
