CAJ | 학술논문

测定了来自江苏省10个市的杂草稻样品的 Rc 基因序列(全长6．4 kb),并整理现有文献中全球166份稻属的 Rc 基因序列.序列共线性分析表明,江苏杂草稻 Rc 基因等位基因类型全部为 Rc 野生型.核苷酸多态性和单倍型分析表明,10份江苏杂草稻 Rc 基因的核苷酸多态性(π＝0．19)和分离位点比率(θw ＝0．28)均高于56份美国杂草稻(π＝0．09；θw ＝0．07).单倍型的进化网络和系统进化树分析表明,江苏杂草稻 Rc 基因并非直接来源于普通白色栽培稻回复突变.此外,发现连云港穞稻和安徽塘稻的 Rc 基因序列与栽培稻相似,而与江苏目前正在发生危害的杂草稻 Rc 基因距离较远.
측정료래자강소성10개시적잡초도양품적 Rc 기인서렬(전장6．4 kb),병정리현유문헌중전구166빈도속적 Rc 기인서렬.서렬공선성분석표명,강소잡초도 Rc 기인등위기인류형전부위 Rc 야생형.핵감산다태성화단배형분석표명,10빈강소잡초도 Rc 기인적핵감산다태성(π＝0．19)화분리위점비솔(θw ＝0．28)균고우56빈미국잡초도(π＝0．09；θw ＝0．07).단배형적진화망락화계통진화수분석표명,강소잡초도 Rc 기인병비직접래원우보통백색재배도회복돌변.차외,발현련운항려도화안휘당도적 Rc 기인서렬여재배도상사,이여강소목전정재발생위해적잡초도 Rc 기인거리교원.
Rc gene(about 6.4 kb)of 10 weedy rice samples from 10 cities in Jiangsu Province was sequenced and 1 66 Rc full-length sequences of Oryza genus in literatures were reviewed.Colinear sequence analysis showed that Rc allele of Jiangsu weedy rice was wild genotype.Nucleotide polymorphism and haplotype analysis showed that Rc nucleotide polymorphism (π=0.1 9)and separation loci ratio (θw =0.28)of 10 weedy rice samples in Jiangsu Province were higher than that of 5 6 weedy rice samples from the United States (π=0.09;θw =0.07).Haplotype network and evolutionary phylogenetic analysis showed that Rc gene of weedy rice in Jiangsu was not directly derived from the revertant mutation of white cultivar.In addition,Rc gene sequences of Ludao from Lianyungang,Jiangsu and Tangdao from Anhui were similar to that of cultivated rice,while genetically distant from weedy rice samples which has been leading to serious infestation in Jiangsu Province.