CAJ | 학술논문

利用籼稻保持系骨干亲本岗46B 与美国水稻品种 Lemont 多次回交培育的低垩白、窄粒株系 K1075与岗46B 构建F2群体,对稻谷粒长、粒宽、长宽比、粒厚、百粒重和垩白粒率进行数量性状位点(QTL)分析.6个性状在 F2群体中均呈正态连续分布,属于典型的数量性状.除粒长与百粒重间没有显著相关性外,其他性状间均存在显著的正相关或负相关.采用SSR 标记构建遗传连锁图,通过复合区间作图法共检测到14个控制稻谷粒形和垩白的 QTL,包括2个控制粒长的 QTL (qGL2和 qGL5),2个控制粒宽的 QTL(qGW5和 qGW8),5个控制长宽比的 QTL(qLWR2、qLWR5、qLWR6、qLWR8和 qL-WR12),1个控制粒厚的 QTL(qGT5),1个控制百粒重的 QTL(qHGW5)和3个控制垩白粒率的 QTL(qPGWC5、qPGWC6和 qPGWC8).在这些位点中,岗46B 等位基因增加粒宽、粒厚、粒重和垩白粒率,减小粒长和长宽比.控制粒宽、粒厚、粒重、垩白粒率和长宽比的主效 QTL 均位于第5染色体 RM17990-RM18004-RM18068区间,这一结果与仅选择极端表型单株,采用连锁及连锁不平衡方法(LD)获得的结果一致.
이용선도보지계골간친본강46B 여미국수도품충 Lemont 다차회교배육적저성백、착립주계 K1075여강46B 구건F2군체,대도곡립장、립관、장관비、립후、백립중화성백립솔진행수량성상위점(QTL)분석.6개성상재 F2군체중균정정태련속분포,속우전형적수량성상.제립장여백립중간몰유현저상관성외,기타성상간균존재현저적정상관혹부상관.채용SSR 표기구건유전련쇄도,통과복합구간작도법공검측도14개공제도곡립형화성백적 QTL,포괄2개공제립장적 QTL (qGL2화 qGL5),2개공제립관적 QTL(qGW5화 qGW8),5개공제장관비적 QTL(qLWR2、qLWR5、qLWR6、qLWR8화 qL-WR12),1개공제립후적 QTL(qGT5),1개공제백립중적 QTL(qHGW5)화3개공제성백립솔적 QTL(qPGWC5、qPGWC6화 qPGWC8).재저사위점중,강46B 등위기인증가립관、립후、립중화성백립솔,감소립장화장관비.공제립관、립후、립중、성백립솔화장관비적주효 QTL 균위우제5염색체 RM17990-RM18004-RM18068구간,저일결과여부선택겁단표형단주,채용련쇄급련쇄불평형방법(LD)획득적결과일치.
To identify the genetic loci that control grain length,width,length-width ratio,thickness,100-grain weight and the percentage of grains with chalkiness (GL,GW,LWR,GT,HGW,PGWC),we constructed an F2 population by crossing an elite indica maintainer line Gang 46B(G46B)with K1075 ,an introgression line of G46B derived from multi-backcross with an US rice variety Lemont, with low percentage of chalkiness and narrow grain.All six investigated traits followed a continuous phenotypic distribution.Significantly positive or negative correlations were observed between all six traits,except the GL and GW.A genetic map was constructed with 30 simple sequence repeat (SSR)markers and a total of 14 QTLs for grain shape and chalkiness were detected by composite interval mapping (CIM).Among which,two were for GL (qGL2 and qGL5 ),two for GW (qGW5 and qGW8 ),five for LWR (qLWR2 ,qLWR5 ,qLWR6 ,qLWR8 and qLWR12 ),one for GT (qGT5 ),one for HGW (qHGW5 ),and three for PGWC (qPGWC5 ,qPGWC6 and qPGWC8 ).The G46B alleles contributed to increasing GW,GT,HGW and PGWC, while decreasing GL and LWR at all detected loci.The major QTLs controlling GW,GT,GW,PGWC and LWR were located in the same region RM1 7990-RM18004-RM18068 on chromosome 5 ,which was correspondent to the results identified in extreme phenotype plants using linkage and linkage disequilibrium.