CAJ | 학술논문

通过60 Co 衰变产生的 r 射线辐照籼稻品种“双科早”,获得小粒密穗突变体 sep1,该突变体与野生型双科早相比表现为籽粒变小,穗轴变短,着粒密度增加.主茎切片观察结果显示,突变体维管束数和单位面积细胞数均比野生型多.遗传分析和等位性分析结果表明,该突变体为隐性突变,与已克隆的直立密穗基因不等位.利用 InDel 分子标记将小粒密穗基因sep1定位在第5染色体着丝粒附近的两个标记 XP26-28与 XP26-31之间,物理距离约为3．896 Mb.此外,还利用该突变体开展了相应的育种利用研究,已获得高代稳定的不育系材料.
통과60 Co 쇠변산생적 r 사선복조선도품충“쌍과조”,획득소립밀수돌변체 sep1,해돌변체여야생형쌍과조상비표현위자립변소,수축변단,착립밀도증가.주경절편관찰결과현시,돌변체유관속수화단위면적세포수균비야생형다.유전분석화등위성분석결과표명,해돌변체위은성돌변,여이극륭적직립밀수기인불등위.이용 InDel 분자표기장소립밀수기인sep1정위재제5염색체착사립부근적량개표기 XP26-28여 XP26-31지간,물리거리약위3．896 Mb.차외,환이용해돌변체개전료상응적육충이용연구,이획득고대은정적불육계재료.
By 60 Co r -ray radiation,a small grain and dense panicle mutant sep1 was obtained from the indica variety Shuangkezao.Compared to the wild type Shuangkezao,it was a mutant with smaller grain,shorter spike-stalk but more grain number per panicle.The histological slice observation of the main stem showed that the mutant had more vascular bundle number in one cycle and more cell number per unit area than the wild type.Genetic and allelism analysis results showed that the small grain and dense panicle mutant was controlled by a recessive gene,and it was different to the dense panicle genes which had been already cloned.Then by constructing the genetic segregated population and using the InDel markers,the small grain and dense panicle gene was mapped on the centromeric region of chromosome 5 between marker XP26-28 and XP26-3 1 .In addition,the breeding application of the mutant was carried out on CMS lines and the stabile lines of high generations had finally been obtained.