农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
3期
449-452,473
,共5页
李余生%杨娟%黄胜东%王才林
李餘生%楊娟%黃勝東%王纔林
리여생%양연%황성동%왕재림
水稻%稻曲病%抗性位点
水稻%稻麯病%抗性位點
수도%도곡병%항성위점
Rice%Rice false smut%Resistance locus
利用157个家系组成的大关稻（japonica）/IR28（indica）重组自交系（Recombinant inbred lines，RIL）群体，采用高效引发稻曲病人工接种方法，以病情指数作为稻曲病的表型值。在南京、扬州分别鉴定了亲本及 RILs对水稻稻曲病的抗性。利用 QTL Cartographer 软件，对水稻稻曲病抗性基因进行检测分析。两个环境下共检测到 qFsr1、qFsr2、qFsr4、qFsr8、qFsr10a、qFsr10b、qFsr11、qFsr12等8个 QTL，分别位于第1、2、4、8、10、11和12染色体上，贡献率在8.6%~22.5%之间。其中，南京检测到qFsr1、qFsr4、qFsr10a、qFsr11、qFsr12等5个位点；扬州检测到 qFsr2、qFsr8、qFsr10a、qFsr10b、qFsr11等5个位点，qFsr10a、qFsr11在两个环境下中均被检测到，对性状的解释率在18.0%~18.9%之间，使病情指数下降8.0%~14.6%,提高了抗病性。根据抗性位点加性效应方向，在qFsr1、qFsr2、qFsr8、qFsr10a、qFsr11和qFsr12位点上，亲本 IR28存在抗稻曲病的增效等位基因，大关稻具有减效等位基因，而位点 qFsr4、qFsr10b的抗性效应来源正好相反。 qFsr10a、qFsr11及其附近的标记可望在稻曲病抗性分子标记辅助选择育种中加以应用。
利用157箇傢繫組成的大關稻（japonica）/IR28（indica）重組自交繫（Recombinant inbred lines，RIL）群體，採用高效引髮稻麯病人工接種方法，以病情指數作為稻麯病的錶型值。在南京、颺州分彆鑒定瞭親本及 RILs對水稻稻麯病的抗性。利用 QTL Cartographer 軟件，對水稻稻麯病抗性基因進行檢測分析。兩箇環境下共檢測到 qFsr1、qFsr2、qFsr4、qFsr8、qFsr10a、qFsr10b、qFsr11、qFsr12等8箇 QTL，分彆位于第1、2、4、8、10、11和12染色體上，貢獻率在8.6%~22.5%之間。其中，南京檢測到qFsr1、qFsr4、qFsr10a、qFsr11、qFsr12等5箇位點；颺州檢測到 qFsr2、qFsr8、qFsr10a、qFsr10b、qFsr11等5箇位點，qFsr10a、qFsr11在兩箇環境下中均被檢測到，對性狀的解釋率在18.0%~18.9%之間，使病情指數下降8.0%~14.6%,提高瞭抗病性。根據抗性位點加性效應方嚮，在qFsr1、qFsr2、qFsr8、qFsr10a、qFsr11和qFsr12位點上，親本 IR28存在抗稻麯病的增效等位基因，大關稻具有減效等位基因，而位點 qFsr4、qFsr10b的抗性效應來源正好相反。 qFsr10a、qFsr11及其附近的標記可望在稻麯病抗性分子標記輔助選擇育種中加以應用。
이용157개가계조성적대관도（japonica）/IR28（indica）중조자교계（Recombinant inbred lines，RIL）군체，채용고효인발도곡병인공접충방법，이병정지수작위도곡병적표형치。재남경、양주분별감정료친본급 RILs대수도도곡병적항성。이용 QTL Cartographer 연건，대수도도곡병항성기인진행검측분석。량개배경하공검측도 qFsr1、qFsr2、qFsr4、qFsr8、qFsr10a、qFsr10b、qFsr11、qFsr12등8개 QTL，분별위우제1、2、4、8、10、11화12염색체상，공헌솔재8.6%~22.5%지간。기중，남경검측도qFsr1、qFsr4、qFsr10a、qFsr11、qFsr12등5개위점；양주검측도 qFsr2、qFsr8、qFsr10a、qFsr10b、qFsr11등5개위점，qFsr10a、qFsr11재량개배경하중균피검측도，대성상적해석솔재18.0%~18.9%지간，사병정지수하강8.0%~14.6%,제고료항병성。근거항성위점가성효응방향，재qFsr1、qFsr2、qFsr8、qFsr10a、qFsr11화qFsr12위점상，친본 IR28존재항도곡병적증효등위기인，대관도구유감효등위기인，이위점 qFsr4、qFsr10b적항성효응래원정호상반。 qFsr10a、qFsr11급기부근적표기가망재도곡병항성분자표기보조선택육충중가이응용。
A recombinant inbred line (RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao (japonica)/IR28 (indica) by the single seed de-scent method was used to detect quantitative trait loci (QTLs) conferring resistance to rice false smut caused by Ustilaginoidea virens(Cooke) Takahashi in Nanjing and Yangzhou. The disease rate index of the two parents and 157 RILs caused by rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software. Eight QTLs control ing false smut resis-tance were detected on chromosomes 1, 2, 4, 8, 10, 11 and 12, respectively, with the phenotypic variance of 8.6%-22.5%. There were five QTLs detected in Nanjing and Yangzhou, respectively, and only two QTLs were found in both two years, the phenotypic variation was explained by individual QTL ranged from 18.0% to 18.9% for these two QTLs, and the additive effects of these two QTLs contributed to the 8.0%-14.6% decrease of disease index and therefore the disease resistance increased. The direction of the additive effects at six loci qFsr1, qFsr2, qFsr8, qFsr10a, qFsr11 and qFsr12 coincided with that predicted by phenotypes of the parents, and the IR28 al eles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao al eles at qFsr4 and qFsr10b. Both qFsr10a and qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection (MAS) program.
