CAJ | 학술논문

[目的]研究一株从土壤中分离得到对鲜绿青霉具有拮抗作用的阿南德氏链霉菌的发酵工艺，探讨培养基组成及发酵条件对其发酵产抗生素的影响。[方法]通过单因素试验和正交试验设计优化培养基组分及发酵条件。[结果]培养基成分和发酵条件对阿南德氏链霉菌产抗生素具有显著影响。最佳培养基配方为可溶性淀粉10 g/L，黄豆饼粉15 g/L， KH2PO40.5 g/L，MgSO4·7H2O 0.1 g/L；最佳发酵条件：发酵温度30℃，培养基初始 pH 7.7，装液量40 ml/250 ml，发酵时间144 h。在最佳的发酵培养基和培养条件下，阿南德氏链霉菌发酵液效价达到452.7 U/ml。[结论]该研究为进一步分离纯化阿南德氏链霉菌产抗鲜绿青霉抗生素奠定了基础。
[목적]연구일주종토양중분리득도대선록청매구유길항작용적아남덕씨련매균적발효공예，탐토배양기조성급발효조건대기발효산항생소적영향。[방법]통과단인소시험화정교시험설계우화배양기조분급발효조건。[결과]배양기성분화발효조건대아남덕씨련매균산항생소구유현저영향。최가배양기배방위가용성정분10 g/L，황두병분15 g/L， KH2PO40.5 g/L，MgSO4·7H2O 0.1 g/L；최가발효조건：발효온도30℃，배양기초시 pH 7.7，장액량40 ml/250 ml，발효시간144 h。재최가적발효배양기화배양조건하，아남덕씨련매균발효액효개체도452.7 U/ml。[결론]해연구위진일보분리순화아남덕씨련매균산항선록청매항생소전정료기출。
Objective] This study aimed to determine the effects of medium composi-tion and fermentation condition on the production of antibiotics from an actinomycete antagonistic to Penicil ium viridicatum, which was isolated from soil and identified primarily as Streptomyces anandi . [Method] Single-factor experiments and orthogonal array designs were used to optimize the medium composition and fermentation con-ditions. [Result] Culture medium composition and fermentation conditions showed sig-nificant influence on the antibiotic production from S. anandi . The optimum culture medium consisted of soluble starch 10 g/L, soybean cake powder 15 g/L, KH2PO4 0.5 g/L and MgSO4·7H2O 0.1 g/L. The optimized fermentation conditions were tem-perature 30 ℃, initial pH value 7.7, culture medium amount 40 ml/250 ml flask, and fermentation time 144 h. The titer of fermentation broth reached 452.7 U/ml when the S. anandi was cultured under the optimal fermentation medium and conditions. [Conclusion] The study may lay the foundation on further isolation and purification of the antibiotic from S. anandi JXNU02.