CAJ | 학술논문

利用氨基功能化离子液体修饰石墨烯( IL-GR)的独特性质,以壳聚糖( CHI)为交联剂,首先在玻碳电极表面固定IL-GR,然后吸附乙酰胆碱酯酶( AChE)制得新型有机磷检测酶电极( AChE/IL-GR/CHI/GCE),并用于卷心菜样品中毒死蜱农药残留的测定。采用透射电镜( TEM)表征了IL-GR的形貌,采用循环伏安法( CV)和差示脉冲伏安法( DPV)研究了酶电极的电化学性质。结果表明,在优化的实验条件下,抑制率(I%)与毒死蜱浓度的对数在1.0×10-10 mol/L~1.0×10-5 mol/L范围内呈良好的线性关系,检出限为7.0×10-12 mol/L(S/N=3)。测定了卷心菜中毒死蜱的含量,回收率为92.3%~108.6%。
이용안기공능화리자액체수식석묵희( IL-GR)적독특성질,이각취당( CHI)위교련제,수선재파탄전겁표면고정IL-GR,연후흡부을선담감지매( AChE)제득신형유궤린검측매전겁( AChE/IL-GR/CHI/GCE),병용우권심채양품중독사비농약잔류적측정。채용투사전경( TEM)표정료IL-GR적형모,채용순배복안법( CV)화차시맥충복안법( DPV)연구료매전겁적전화학성질。결과표명,재우화적실험조건하,억제솔(I%)여독사비농도적대수재1.0×10-10 mol/L~1.0×10-5 mol/L범위내정량호적선성관계,검출한위7.0×10-12 mol/L(S/N=3)。측정료권심채중독사비적함량,회수솔위92.3%~108.6%。
According to the special properties of functionalized ionic liquid modified graphene( IL-GR) ,IL-GR was first immobilized on the glass carbon electrode via chitosan ( CHI ) cross-linking, Finally, acetylcholinesterase ( AChE) can be immobilized on the composite film by adsorption. A novel biosensor for detecting organophosphorus pesticides enzyme biosensor ( AChE/IL-GR/CHI/GCE ) was developed and was used for the determination of chlorpyrifos. Different technologies were employed to study the construction process and the electrochemical properties of the biosensor. Linear relationship between the inhibition percentage ( I%) and logarithm of concentration of chlorpyrifos was found in the range of 1. 0í10-10 mol/L~1. 0í10-5 mol/L,and the detection limit was calculated to be about 7 . 0í10-12 mol/L ( S/N=3 ) . The recoveries of chlorpyrifos in vegetable were from 92.3% to 108 . 6%.