CAJ | 학술논문

针对西瓜炭疽病菌进行了产毒条件优化、粗毒素的生物活性及其理化性质测定的研究。结果表明：不同的培养条件对病原菌粗毒素活性的影响不同，pH值为6的Richard培养液产生的粗毒素的生物活性最强，培养温度25℃、黑暗条件下持续振荡培养20 d为最佳产毒条件。粗毒素接种西瓜茎蔓可产生与病菌作用相同的发病症状，对种子胚根生长及种子萌发有抑制作用，对幼苗有致萎作用，说明该粗毒素是西瓜炭疽病菌侵染过程中的主要致病因子。以病菌粗毒素接种10种供试植物，其中甜瓜、西葫芦及草莓发病症状明显，而番茄、茄子及辣椒均未见发病症状。
침대서과탄저병균진행료산독조건우화、조독소적생물활성급기이화성질측정적연구。결과표명：불동적배양조건대병원균조독소활성적영향불동，pH치위6적Richard배양액산생적조독소적생물활성최강，배양온도25℃、흑암조건하지속진탕배양20 d위최가산독조건。조독소접충서과경만가산생여병균작용상동적발병증상，대충자배근생장급충자맹발유억제작용，대유묘유치위작용，설명해조독소시서과탄저병균침염과정중적주요치병인자。이병균조독소접충10충공시식물，기중첨과、서호호급초매발병증상명현，이번가、가자급랄초균미견발병증상。
Toxin-producing condition optimization and determination of crude toxin biological activity and its physical,chemical properties were carried out on Colletotrichum orbiculare. The results showed that different culture conditions had different influences on pathogenic toxin activity. The Richard culture medium with pH 6 ,culture temperature at 25℃,in dark,and shaking continuously for 20 d were the best toxin-producing conditions. The watermelon stem inoculated by crude toxin could produce the same acute symptom as the bacteria did,which had inhibitory effect on seed radicle growth and seed germination; and also a collapsing role on seedling. That toxin is the main pathogenic factor in the process of watermelon anthrax bacteria infection. Among the 10 kinds of selected plants inoculated by crude toxin,the symptoms of muskmelon,summer squash and strawberry were significant,while no symptoms were seen on tomato,eggplant and pepper.