CAJ | 학술논문

目的：探讨不同剂量单纯照射及照射联合吉非替尼对非小细胞肺癌细胞系 H358存活率、增敏比及细胞凋亡、细胞周期的影响。方法体外培养肺腺癌细胞 H358分为空白对照组、单纯照射组、单纯吉非替尼（Iressa）组、照射＋Iressa组，照射剂量为0、2、4、6、8、12、16、20 Gy，药物浓度为1μmol/L。通过细胞克隆形成实验，观察4组 H358细胞存活情况，描绘细胞存活曲线；采用四噻唑比色试验（MTT）观察两组细胞生长受抑制情况，计算增敏比（SER）；运用流式细胞仪检测细胞凋亡率和细胞周期。结果（1）随着照射剂量增加细胞存活分数减小，单纯照射组单次剂量达到20 Gy时，细胞克隆集落形成率为0，照射＋Iressa组单次剂量达到16 Gy时克隆集落形成率为0；（2）照射＋Iressa 组与单纯照射组相比，两组 OD 值差异有统计学意义（F剂量＝62．644，P ＜0．001，F药物＝233．572，P ＜0．001），两组 OD值随着照射剂量的增高而减小，不同剂量之间 OD值差异有统计学意义（F未加药＝354．972，P＜0．001；F加药＝231．740，P ＜0．001），两组细胞放射增敏比（SER）与药物显著相关（P ＜0．001），照射＋Iressa组SER较单纯照射组明显增高；（3）随着照射剂量的增加，凋亡率增高（P ＜0．001），H358细胞凋亡率与Iressa药物作用相关（P ＜0．001），单纯Iressa作用后细胞周期阻滞主要出现在G0/G1期，照射＋Iressa组及单纯照射组主要出现G2/M期阻滞。结论增加单次照射剂量可降低 H358细胞存活率，细胞凋亡与 Iressa药物之间存在相关性，照射＋Iressa能够增加细胞凋亡率，照射前使用Iressa能够增强照射对细胞的放射敏感性。
목적：탐토불동제량단순조사급조사연합길비체니대비소세포폐암세포계 H358존활솔、증민비급세포조망、세포주기적영향。방법체외배양폐선암세포 H358분위공백대조조、단순조사조、단순길비체니（Iressa）조、조사＋Iressa조，조사제량위0、2、4、6、8、12、16、20 Gy，약물농도위1μmol/L。통과세포극륭형성실험，관찰4조 H358세포존활정황，묘회세포존활곡선；채용사새서비색시험（MTT）관찰량조세포생장수억제정황，계산증민비（SER）；운용류식세포의검측세포조망솔화세포주기。결과（1）수착조사제량증가세포존활분수감소，단순조사조단차제량체도20 Gy시，세포극륭집락형성솔위0，조사＋Iressa조단차제량체도16 Gy시극륭집락형성솔위0；（2）조사＋Iressa 조여단순조사조상비，량조 OD 치차이유통계학의의（F제량＝62．644，P ＜0．001，F약물＝233．572，P ＜0．001），량조 OD치수착조사제량적증고이감소，불동제량지간 OD치차이유통계학의의（F미가약＝354．972，P＜0．001；F가약＝231．740，P ＜0．001），량조세포방사증민비（SER）여약물현저상관（P ＜0．001），조사＋Iressa조SER교단순조사조명현증고；（3）수착조사제량적증가，조망솔증고（P ＜0．001），H358세포조망솔여Iressa약물작용상관（P ＜0．001），단순Iressa작용후세포주기조체주요출현재G0/G1기，조사＋Iressa조급단순조사조주요출현G2/M기조체。결론증가단차조사제량가강저 H358세포존활솔，세포조망여 Iressa약물지간존재상관성，조사＋Iressa능구증가세포조망솔，조사전사용Iressa능구증강조사대세포적방사민감성。
Objective This study aimed to explore the effects on the survival,sensitization enhancement ra-tio and cell apoptosis,cell cycle in non-small cell lung cancer cell lines H358 by a single different dose of irradiation and the irradiation combined with gefitinib.Methods Lung adenocarcinoma cells H358 in vitro were divided into four groups:control group,irradiation group,Iressa group,irradiation combined Iressa group,with dose 0-20 Gy and drug concentration 1μmol/L.Observe H358 cell survival by cell colony formation assay and depict cell survival curves;Observe cell growth inhibition by MTT and calculate SER;Detect cell apoptosis and cell cycle by flow cytometry;Process data by SPSS17.0.Results Cell colony for-mation assay showed that cell survival fraction decreased with the increase of irradiation dose,when a sin-gle dose irradiation reached 20Gy,cell clone formation rate was 0;in irradiation combined Iressa group, clonal colony formation rate was 0 when the dose reached 1 6 Gy.MTT results showed that by comparing Iressa combined irradiation group and simple irradiation group,there were significant differences between OD values in the two groups (Fdose=62.644,P<0.001,Fdrug=233.572,P<0.001),two groups of OD val-ues decreased as the dose increased;OD value differences in different doses were statistically significant (Fnodrug=354.972,P<0.001;Fdrug=231.740,P<0.001),two groups cell radiosensitization ratio (SER) were significantly associated with the drug (P <0.001),SER of Iressa radiation group was significantly higher than that in the irradiation group;The result of flow cytometry showed that H358 cell apoptosis were associated with Iressa drug effects (P <0.001),H358 cell apoptosis rate increased with the increase of single dose (P <0.001).Simple Iressa group cell cycle arrest mainly appeared in G0/G1 phase,Iressa combined irradiation group and simple irradiation group mainly in G2/M phase.Conclusion Increased sin-gle dose decreased H358 cell survival;There was a correlation between cell apoptosis and the drug Iressa;Irradiation combined Iressa group increased the rate of cell apoptosis;Used Iressa before irradiation en-hanced the radiosensitivity of irradiation to cells.