按摩与康复医学
按摩與康複醫學
안마여강복의학
Chinese Manipulation & Rehabilitation Medicine
2014年
5期
25-27,28
,共4页
醒脑静注射液%PC12细胞%凋亡%GABAA受体%相关性
醒腦靜註射液%PC12細胞%凋亡%GABAA受體%相關性
성뇌정주사액%PC12세포%조망%GABAA수체%상관성
xingnaojing injection%PC12 cells,apoptosis%GABAA receptor%correlation
目的：研究GABAA受体是否参与醒脑静注射液抗神经细胞凋亡的作用。方法：采用血清饥饿3天的方法建立PC12细胞凋亡模型，并随机分为正常对照组（control组）、模型组（model组）、醒脑静组（XNJ组），GABAA受体阻断剂组（BIC组）、醒脑静加GABAA受体阻断剂组（XNJ+BIC组）。正常对照组以含10%FBS的正常培养基培养，不作任何处理；模型组换成无血清培养基培养；醒脑静组在无血清培养基基础上加入1.5μL/mL醒脑静注射液；BIC组加入终浓度为200μmol/mL的荷包牡丹碱；XNJ+BIC组同时加入醒脑静注射液1.5μL/mL和终浓度为200μmol/mL的荷包牡丹碱；每组5个复孔，采用Annexin V/PI双染流式细胞仪检测神经细胞凋亡数，用Western Blotting检测GABAA受体的蛋白表达。结果：XNJ+BIC组凋亡少于模型组（P<0.05）；醒脑静注射液加入GABAA受体阻断剂后其GABAA受体蛋白表达较醒脑静注射液降低。结论：醒脑静注射液抗细胞凋亡的作用机制与提高GABAA受体从而抑制凋亡效应有关。
目的：研究GABAA受體是否參與醒腦靜註射液抗神經細胞凋亡的作用。方法：採用血清饑餓3天的方法建立PC12細胞凋亡模型，併隨機分為正常對照組（control組）、模型組（model組）、醒腦靜組（XNJ組），GABAA受體阻斷劑組（BIC組）、醒腦靜加GABAA受體阻斷劑組（XNJ+BIC組）。正常對照組以含10%FBS的正常培養基培養，不作任何處理；模型組換成無血清培養基培養；醒腦靜組在無血清培養基基礎上加入1.5μL/mL醒腦靜註射液；BIC組加入終濃度為200μmol/mL的荷包牡丹堿；XNJ+BIC組同時加入醒腦靜註射液1.5μL/mL和終濃度為200μmol/mL的荷包牡丹堿；每組5箇複孔，採用Annexin V/PI雙染流式細胞儀檢測神經細胞凋亡數，用Western Blotting檢測GABAA受體的蛋白錶達。結果：XNJ+BIC組凋亡少于模型組（P<0.05）；醒腦靜註射液加入GABAA受體阻斷劑後其GABAA受體蛋白錶達較醒腦靜註射液降低。結論：醒腦靜註射液抗細胞凋亡的作用機製與提高GABAA受體從而抑製凋亡效應有關。
목적：연구GABAA수체시부삼여성뇌정주사액항신경세포조망적작용。방법：채용혈청기아3천적방법건립PC12세포조망모형，병수궤분위정상대조조（control조）、모형조（model조）、성뇌정조（XNJ조），GABAA수체조단제조（BIC조）、성뇌정가GABAA수체조단제조（XNJ+BIC조）。정상대조조이함10%FBS적정상배양기배양，불작임하처리；모형조환성무혈청배양기배양；성뇌정조재무혈청배양기기출상가입1.5μL/mL성뇌정주사액；BIC조가입종농도위200μmol/mL적하포모단감；XNJ+BIC조동시가입성뇌정주사액1.5μL/mL화종농도위200μmol/mL적하포모단감；매조5개복공，채용Annexin V/PI쌍염류식세포의검측신경세포조망수，용Western Blotting검측GABAA수체적단백표체。결과：XNJ+BIC조조망소우모형조（P<0.05）；성뇌정주사액가입GABAA수체조단제후기GABAA수체단백표체교성뇌정주사액강저。결론：성뇌정주사액항세포조망적작용궤제여제고GABAA수체종이억제조망효응유관。
Objective:To elucidate the role of GABAA receptor in the protective effect of xingnaojing injection on neurons apoptosis. Methods:The PC12 apoptosis model was established by serum starvation for 3 days. The cells were randomly classified into four groups: control group, model group, xingnaojing group (XNJ group), GABAA receptor antagonist Group (BIC group), xingnaojin and GABAA receptor antagonist Group (XNJ+BIC group). The control group only cultured with 10%FBS of normal medium;model group treated with serum-free culturel;XNJ group joined for 1.5μL/mL xingnaojing injection after the serum-free culturel. BIC Group joined 200μmoL/mL bicuculline;XNJ+BIC group joined 1.5μL/mL xing-naojing injection and 200μmoL/mL bicuculline. Each group has 5 double wells. Ratio of cell apoptosis was examined by Annexin V/PI double stain flow cytometry (FCM), and Western blot was applied to detect the expression of GABAA receptor protein. Results:XNJ+BIC group compared with model group, the apoptosis is significantly decreased (P<0.05). The expression of GABAA is significantly decreased by the combination of xingnao-jing injection and GABAA receptor antagonist bicuculline. Conclusion:The mechanisms of xingnaojing injection protection against cell apoptosis are related to the anti-apoptosis effect of GABAA receptor.
