CAJ | 학술논문

探讨不同浓度维生素C和培养时间对小鼠体细胞核移植胚胎发育潜能的影响。不同浓度维生素 C (0、50、100 mg/L)分别处理小鼠核移植重构胚,50 mg/L维生素C浓度组处理重构胚18 h,获得的囊胚率以及胚胎着床率显著高于其他组(P<0.05),且在此基础上提高维生素C浓度或处理时间不能进一步提高克隆胚的发育潜能( P>0.05)。小鼠体细胞核移植胚胎培养时,适当的维生素C浓度和培养时间可显著提高小鼠重构胚的发育潜能。
탐토불동농도유생소C화배양시간대소서체세포핵이식배태발육잠능적영향。불동농도유생소 C (0、50、100 mg/L)분별처리소서핵이식중구배,50 mg/L유생소C농도조처리중구배18 h,획득적낭배솔이급배태착상솔현저고우기타조(P<0.05),차재차기출상제고유생소C농도혹처리시간불능진일보제고극륭배적발육잠능( P>0.05)。소서체세포핵이식배태배양시,괄당적유생소C농도화배양시간가현저제고소서중구배적발육잠능。
To investigate the impact of different concentrations and time of vitamin C on mouse somatic cell nuclear transfer embryo development potential. Mice reconstructed embryos were treated with different concentrations (0, 50, 100 mg/L) of vitamin C, the blastocyst and implantation rate was significantly higher than the other groups when this embryos were treated with 50 mg/L concentrations of vitamin C for 18 h ( P<0.05 ) , on the basis of this condition,developmental potential of cloned embryos could not be further enhanced either by increasing the concen-tration of vitamin C or by prolonging the processing time ( P>0.05 ) . Appropriate concentrations and incubation time of vitamin C could significantly improve the developmental potential of reconstructed embryos in the process of mouse somatic cell nuclear transfer.