安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
5期
653-657,658
,共6页
李小萍%王巧民%褚源%宋继中%沈佐君
李小萍%王巧民%褚源%宋繼中%瀋佐君
리소평%왕교민%저원%송계중%침좌군
肠易激综合征%肠道菌群%实时荧光定量PCR
腸易激綜閤徵%腸道菌群%實時熒光定量PCR
장역격종합정%장도균군%실시형광정량PCR
irritable bowel syndrome%intestinal flora%real-time fluorescence quantitative PCR
目的研究腹泻型肠易激综合征( IBS-D )患者与正常对照者的肠道菌群差异。方法实时荧光定量PCR法检测50例IBS-D患者及25例正常对照者粪便中长双歧杆菌属、乳酸杆菌属、脆弱拟杆菌、产气荚膜梭菌、大肠杆菌和粪肠球菌属的数量,并对各组的目标菌群数量进行比较,计算肠道定值抗力,肠道定值抗力( CR)是指肠道内需氧的潜在致病菌群被肠道内源性厌氧菌抑制的能力,双歧杆菌数值与肠杆菌数值之比作为肠道微生物定值抗力的指标,即 B/E值。结果与正常对照者比较,IBS-D患者粪便中大肠埃希菌、产气荚膜梭菌的数量明显增多(P<0.05),而乳酸及双歧杆菌属的数量明显减少(P<0.05),粪肠球菌、脆弱拟杆菌在两组之间比较差异无统计学意义。 IBS-D患者B/E值<1,与正常对照者比较明显降低(P<0.05)。结论IBS-D患者肠道菌群平衡被打破,表现为B/E值降低,且粪便中肠杆菌及产气荚膜梭菌数量增加,双歧杆菌、乳酸杆菌的数量明显减少。
目的研究腹瀉型腸易激綜閤徵( IBS-D )患者與正常對照者的腸道菌群差異。方法實時熒光定量PCR法檢測50例IBS-D患者及25例正常對照者糞便中長雙歧桿菌屬、乳痠桿菌屬、脆弱擬桿菌、產氣莢膜梭菌、大腸桿菌和糞腸毬菌屬的數量,併對各組的目標菌群數量進行比較,計算腸道定值抗力,腸道定值抗力( CR)是指腸道內需氧的潛在緻病菌群被腸道內源性厭氧菌抑製的能力,雙歧桿菌數值與腸桿菌數值之比作為腸道微生物定值抗力的指標,即 B/E值。結果與正常對照者比較,IBS-D患者糞便中大腸埃希菌、產氣莢膜梭菌的數量明顯增多(P<0.05),而乳痠及雙歧桿菌屬的數量明顯減少(P<0.05),糞腸毬菌、脆弱擬桿菌在兩組之間比較差異無統計學意義。 IBS-D患者B/E值<1,與正常對照者比較明顯降低(P<0.05)。結論IBS-D患者腸道菌群平衡被打破,錶現為B/E值降低,且糞便中腸桿菌及產氣莢膜梭菌數量增加,雙歧桿菌、乳痠桿菌的數量明顯減少。
목적연구복사형장역격종합정( IBS-D )환자여정상대조자적장도균군차이。방법실시형광정량PCR법검측50례IBS-D환자급25례정상대조자분편중장쌍기간균속、유산간균속、취약의간균、산기협막사균、대장간균화분장구균속적수량,병대각조적목표균군수량진행비교,계산장도정치항력,장도정치항력( CR)시지장도내수양적잠재치병균군피장도내원성염양균억제적능력,쌍기간균수치여장간균수치지비작위장도미생물정치항력적지표,즉 B/E치。결과여정상대조자비교,IBS-D환자분편중대장애희균、산기협막사균적수량명현증다(P<0.05),이유산급쌍기간균속적수량명현감소(P<0.05),분장구균、취약의간균재량조지간비교차이무통계학의의。 IBS-D환자B/E치<1,여정상대조자비교명현강저(P<0.05)。결론IBS-D환자장도균군평형피타파,표현위B/E치강저,차분편중장간균급산기협막사균수량증가,쌍기간균、유산간균적수량명현감소。
Objective To study on intestinal flora in diarrhea predominant irritable bowel syndrome ( IBS-D) with differences between normal people. Methods The real-time fluorescence quantitative PCR assay was used in 50 patients with diarrhea predominant IBS and 25 normal control subjects in feces to detect Bifidobacteria longum, Lactobacillus, Bacteroides, Clostridium perfringens, Escherichia coli and Enterococcus faecalis number. The target bacteria in the number in each group were compared, and calculated intestinal colonization resistance. Intestinal re-sistance value ( coloniza-tion resistance, CR) was a potential aerobic intestine by intestinal pathogens in anaerobic inhibition ability of endogenous bifidobacteria value and the value as the ratio of intestinal Enterobacteriaceae micro-bial resistance value of the index, namely B/E values. Results Compared with the normal control group, feces of patients with diarrhea predominant IBS in numbers of Escherichia coli and Clostridium perfringens increased signifi-cantly ( P<0.05 ) , while the number of Lactobacillus and Bifidobacteria decreased significantly ( P<0.05 ) , no statistically significant difference between the two groups in Enterococcus faecalis and Bacteroides fragilis. Patients with intestinal diarrhea predominant IBS constant force B/E<1 , compared with the control group decreased signifi-cantly (P<0.05). Conclusion Diarrhea predominant IBS patients of intestinal flora balance is broken, for gut setting value is reduced, and the feces of Escherichia coli and Clostridium perfringens increase in number, number of Bifidobacterium, Lactobacillus decreased.