CAJ | 학술논문

目的动态观察急性脑梗死患者不同时期外周血单个核细胞( PBMC )中 CD4+ CD25+ CD127 low调节性 T 细胞( Treg)表达，探讨其对急性缺血性脑卒中免疫状态及病程进展的影响。方法选取急性脑梗死患者60例为脑卒中组，并分为发病24~48 h组( n=16)、3~7 d组( n=22)和8~14 d组(n=22)；另选取健康体检者22例为对照组。采用流式细胞术检测60例急性脑梗死患者和22例健康体检者外周血中CD4+CD25+CD127 low Treg水平并进行分析。结果与对照组比较，脑卒中组外周血 CD4+ CD25+ CD127 low、CD4+CD25 high Treg比例在起病后24~48 h、3~7 d时显著降低(P<0.01)；8~14 d逐渐恢复至正常水平，与对照组比较差异无统计学意义( P>0.05)；与对照组比较，脑卒中组的CD4+T细胞比例升高，差异有统计学意义( P<0.05)。结论Treg可能参与急性缺血性脑卒中的病理生理过程，其功能失衡在急性缺血性脑卒中炎性损伤中具有重要作用，早期检测可能为其治疗提供依据。
목적동태관찰급성뇌경사환자불동시기외주혈단개핵세포( PBMC )중 CD4+ CD25+ CD127 low조절성 T 세포( Treg)표체，탐토기대급성결혈성뇌졸중면역상태급병정진전적영향。방법선취급성뇌경사환자60례위뇌졸중조，병분위발병24~48 h조( n=16)、3~7 d조( n=22)화8~14 d조(n=22)；령선취건강체검자22례위대조조。채용류식세포술검측60례급성뇌경사환자화22례건강체검자외주혈중CD4+CD25+CD127 low Treg수평병진행분석。결과여대조조비교，뇌졸중조외주혈 CD4+ CD25+ CD127 low、CD4+CD25 high Treg비례재기병후24~48 h、3~7 d시현저강저(P<0.01)；8~14 d축점회복지정상수평，여대조조비교차이무통계학의의( P>0.05)；여대조조비교，뇌졸중조적CD4+T세포비례승고，차이유통계학의의( P<0.05)。결론Treg가능삼여급성결혈성뇌졸중적병리생리과정，기공능실형재급성결혈성뇌졸중염성손상중구유중요작용，조기검측가능위기치료제공의거。
Objective To investigate the expression of CD4 +CD25 +CD127 low regulatory T cells in peripheral blood mononuclear cells( PBMNCs) in order to explore the influence on the immune status and disease progression in the different period of acute ischemic stroke. Methods 60 patients with acute cerebral infarction were selected as the stroke group,and divided them into the 24~48 h group (n=16), 3~7 d group (n=22) and 8~14 d group (n=22);22 healthy human were set as the control group. To analyze the percentage of CD4 +CD25 +CD127low regu-latory T cells in the peripheral blood of acute cerebral infarction patients and healthy human with flow cytometry. Results The percentages of CD4 +CD25 +CD127 low and CD4 +CD25 high regulatory T cells in the peripheral blood of the stroke group were significantly decreased at 24 ~48 h,3 ~7 d ( P <0.01 ) when compared with the control group, and then tended to return toward the control value at 8~14 d (P>0.05);the percentage of CD4 + regula-tory T cells in the peripheral blood of the stroke group was significantly higher than control group ( P <0.05 ) . Conclusion Imbalance of regulatory T cells is very likely to play an important role in the immunological injury of acute ischemic stroke. Regulatory T cells may be involved in the pathogenesis of acute ischemic stroke,and early detection may provide a basis for treatment.