安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
5期
625-628
,共4页
陈佳%余宏铸%王正林%张晓亮
陳佳%餘宏鑄%王正林%張曉亮
진가%여굉주%왕정림%장효량
胆管细胞癌%TLR4%NF-κB%肝内胆管结石
膽管細胞癌%TLR4%NF-κB%肝內膽管結石
담관세포암%TLR4%NF-κB%간내담관결석
cholangiocarcinoma%TLR4%NF-κB%intrahepatic
目的探讨Toll样受体4( TLR4)和核因子κB ( NF-κB)在肝内胆管结石相关的肝内胆管细胞癌组织中的表达及其临床意义。方法收集110例胆管病理组织蜡块，包括40例肝内胆管结石相关的肝内胆管细胞癌组织、40例肝内胆管结石旁胆管组织和30例正常肝内胆管组织(肝血管瘤旁正常胆管组织或者肝外伤后切除肝组织的正常胆管组织)，采用免疫组织化学( PV)法检测各组织中TLR4和NF-κB的表达情况并分析结果。结果肝内胆管结石相关的肝内胆管细胞癌组织中的TLR4和NF-κB表达量明显高于肝内胆管结石旁胆管组织和正常肝内胆管组织( P <0.05)。在肝内胆管结石相关的肝内胆管细胞癌组织中TLR4和NF-κB的表达呈正相关( r=0.709)。 TLR4和NF-κB的表达与患者的性别、年龄、肿瘤分化程度及淋巴结转移的组间比较，差异无统计学意义(P>0.05)。结论TLR4和NF-κB的表达与肝内胆管结石到肝内胆管结石相关的肝内胆管细胞癌的逐步发展具有密切相关性，且两者表达呈正相关。
目的探討Toll樣受體4( TLR4)和覈因子κB ( NF-κB)在肝內膽管結石相關的肝內膽管細胞癌組織中的錶達及其臨床意義。方法收集110例膽管病理組織蠟塊，包括40例肝內膽管結石相關的肝內膽管細胞癌組織、40例肝內膽管結石徬膽管組織和30例正常肝內膽管組織(肝血管瘤徬正常膽管組織或者肝外傷後切除肝組織的正常膽管組織)，採用免疫組織化學( PV)法檢測各組織中TLR4和NF-κB的錶達情況併分析結果。結果肝內膽管結石相關的肝內膽管細胞癌組織中的TLR4和NF-κB錶達量明顯高于肝內膽管結石徬膽管組織和正常肝內膽管組織( P <0.05)。在肝內膽管結石相關的肝內膽管細胞癌組織中TLR4和NF-κB的錶達呈正相關( r=0.709)。 TLR4和NF-κB的錶達與患者的性彆、年齡、腫瘤分化程度及淋巴結轉移的組間比較，差異無統計學意義(P>0.05)。結論TLR4和NF-κB的錶達與肝內膽管結石到肝內膽管結石相關的肝內膽管細胞癌的逐步髮展具有密切相關性，且兩者錶達呈正相關。
목적탐토Toll양수체4( TLR4)화핵인자κB ( NF-κB)재간내담관결석상관적간내담관세포암조직중적표체급기림상의의。방법수집110례담관병리조직사괴，포괄40례간내담관결석상관적간내담관세포암조직、40례간내담관결석방담관조직화30례정상간내담관조직(간혈관류방정상담관조직혹자간외상후절제간조직적정상담관조직)，채용면역조직화학( PV)법검측각조직중TLR4화NF-κB적표체정황병분석결과。결과간내담관결석상관적간내담관세포암조직중적TLR4화NF-κB표체량명현고우간내담관결석방담관조직화정상간내담관조직( P <0.05)。재간내담관결석상관적간내담관세포암조직중TLR4화NF-κB적표체정정상관( r=0.709)。 TLR4화NF-κB적표체여환자적성별、년령、종류분화정도급림파결전이적조간비교，차이무통계학의의(P>0.05)。결론TLR4화NF-κB적표체여간내담관결석도간내담관결석상관적간내담관세포암적축보발전구유밀절상관성，차량자표체정정상관。
Objective To investigate the expressions and clinical significance of NF-κB and TLR4 in intrahepatic lithiasis associated with intrahepatic cholangiocarcinoma. Methods Collect bile duct histopathologic wax blocks of liver tissues specimen of hepatectomy which were selected in department of pathology of the first affiliated hospital of Anhui medical university in recent 10 years, including 40 cases of intrahepatic bile duct calculi of intrahepatic bile duct carcinoma tissue,40 cases of intrahepatic biliary calculi by bile duct tissues and 30 cases of normal bile duct tissues( normal bile duct tissues adjacent to liver hemangioma or liver trauma after resection of liver tissue of normal bile duct tissues) . Immune histochemical method ( PV) was tested in organizations in NF-κB and the expression of TLR4 and results were analyzed. Results The amount of NF-κB and TLR4 expression in intrahepatic biliary calcu-li of intrahepatic bile duct carcinoma tissue was significantly higher than that in the intrahepatic bile duct stones near bile duct tissues and normal bile duct tissue ( P<0.05 ) . The expression of TLR4 and NF-κB in intrahepatic biliary calculi of intrahepatic bile duct carcinoma was correlative and presents positive correlation(r=0.709). The comparison between groups of patients’age, sex, tumor differentiation, tumor invasion and lymph node metastasis that related the expression of TLR4 and NF-κB showed that there was no statistically significant difference ( P >0.05 ) . Conclusion The abnormal expression of TLR4 and NF-κB is closely associated with the gradual progress of intrahepatic bile duct stones complicated with intrahepatic bile duct carcinoma, and both are positively correla-ted.
