CAJ | 학술논문

目的研究大鼠肺缺血再灌注损伤后血清炎症因子及肺组织相关酶活性的变化，并探讨外源性瘦素( Leptin)在肺缺血损伤中的作用及可能机制。方法建立大鼠肺缺血再灌注损伤模型，并用外源性Leptin干预治疗，对各组术后肺组织匀浆中丙二醛( MDA)、髓过氧化物酶( MPO)、超氧化物歧化酶( SOD)的活性、血清C反应蛋白( CRP)、肿瘤坏死因子-α( TNF-α)和核因子-资B ( NF-资B )浓度进行分析和比较，并观察各组肺组织病理学改变。结果缺血再灌注组血清炎症指标CRP、TNF-α、NF-资B浓度较假手术组明显升高，用外源性Leptin预处理后的炎症指标浓度较缺血再灌注组有显著下降( P<0.05)。肺组织匀浆MPO、MDA水平测定结果同样显示Leptin预处理组的肺组织匀浆酶学指标较缺血再灌注组有显著下降( P<0.05)。组织学病理显示缺血再灌注组肺损伤严重；Leptin预处理组肺泡结构较完整，炎症改变相对较轻，水肿及出血较再灌注组明显减少。结论运用外源性Leptin后肺缺血再灌注损伤明显减轻；外源性Leptin对肺缺血再灌注损伤有保护作用，可能与减轻脂质过氧化反应及炎性细胞浸润有关。
목적연구대서폐결혈재관주손상후혈청염증인자급폐조직상관매활성적변화，병탐토외원성수소( Leptin)재폐결혈손상중적작용급가능궤제。방법건립대서폐결혈재관주손상모형，병용외원성Leptin간예치료，대각조술후폐조직균장중병이철( MDA)、수과양화물매( MPO)、초양화물기화매( SOD)적활성、혈청C반응단백( CRP)、종류배사인자-α( TNF-α)화핵인자-자B ( NF-자B )농도진행분석화비교，병관찰각조폐조직병이학개변。결과결혈재관주조혈청염증지표CRP、TNF-α、NF-자B농도교가수술조명현승고，용외원성Leptin예처리후적염증지표농도교결혈재관주조유현저하강( P<0.05)。폐조직균장MPO、MDA수평측정결과동양현시Leptin예처리조적폐조직균장매학지표교결혈재관주조유현저하강( P<0.05)。조직학병리현시결혈재관주조폐손상엄중；Leptin예처리조폐포결구교완정，염증개변상대교경，수종급출혈교재관주조명현감소。결론운용외원성Leptin후폐결혈재관주손상명현감경；외원성Leptin대폐결혈재관주손상유보호작용，가능여감경지질과양화반응급염성세포침윤유관。
Objective Rats serum inflammatory factors and enzyme activities of lung tissue were measured to inves-tigate the protective role and mechanisms of exogenous Leptin alleviating rat lung ischemia-reperfusion injury. Methods Rats lung ischemia-reperfusion injury models were established and one group was treated with exogenous Leptin. Plasma concentrations of C-reactive protein ( CRP) , tumor necrosis factor-α ( TNF-α) and nuclear factor-κB ( NF-κB) were measured by ELISA. The activity of malondialdehyde ( MDA) , myeloperoxidase ( MPO) , su-peroxide dismutase ( SOD) of lung homogenates were measured using colorimetric assay, and the morphological changes were observed under a microscope. Results In ischemia-reperfusion group, CRP, TNF-α, NF-κB con-centration were significantly higher than that of the sham group, and the concentration of exogenous Leptin group was significantly decreased compared with the value of ischemia-reperfusion group ( P<0.05 ) . The lung tissue enzymatic indicators examination also showed that the MPO, MDA levels of exogenous Leptin pretreatment had sig-nificantly decreased ( P<0.05 ) compared with ischemia-reperfusion group ( P<0.05 ) . Histological study showed relatively mild inflammation, edema and bleeding in exogenous Leptin treatment group with alveolar structure intact compared with ischemia-reperfusion injury group. Conclusion Lung ischemia-reperfusion injury was significantly alleviated after application of exogenous Leptin. The exogenous Leptin may play a protective role in lung ischemia-reperfusion injury through reducing lipid peroxidation and inflammatory cell infiltration.