安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
5期
598-602
,共5页
宋旆文%徐鹏%尤涛%董福龙%章仁杰%申才良
宋旆文%徐鵬%尤濤%董福龍%章仁傑%申纔良
송패문%서붕%우도%동복룡%장인걸%신재량
间充质干细胞%神经干细胞%分化%条件培养基
間充質榦細胞%神經榦細胞%分化%條件培養基
간충질간세포%신경간세포%분화%조건배양기
mesenchymal stem cell%neural stem cell%differentiation%conditioned medium
目的观察骨髓间充质干细胞( BMSCs)条件培养基对神经干细胞( NSCs)分化的影响。方法提取第3代大鼠BMSCs条件培养基,浓缩后加到NSCs培养基中,对所培养的NSCs进行形态学观察和免疫化学染色法鉴定。结果在加入大鼠BMSCs条件培养基的实验组,24 h内见悬浮生长的NSCs球开始贴壁生长。至第3天,所有悬浮细胞球基本贴壁生长,大量细胞从中爬出,伸出突起。第7天时,伸出细胞交织成网。对贴壁分化后的NSCs进行免疫化学染色,其表达神经元和胶质细胞的特异性蛋白。而未加入条件培养基的对照组,NSCs仍呈悬浮生长,未见大量细胞贴壁生长及分化。结论在完全去除BMSCs细胞的情况下,含有大鼠BMSCs分泌的细胞因子的条件培养基对NSCs的贴壁分化有显著的促进作用。
目的觀察骨髓間充質榦細胞( BMSCs)條件培養基對神經榦細胞( NSCs)分化的影響。方法提取第3代大鼠BMSCs條件培養基,濃縮後加到NSCs培養基中,對所培養的NSCs進行形態學觀察和免疫化學染色法鑒定。結果在加入大鼠BMSCs條件培養基的實驗組,24 h內見懸浮生長的NSCs毬開始貼壁生長。至第3天,所有懸浮細胞毬基本貼壁生長,大量細胞從中爬齣,伸齣突起。第7天時,伸齣細胞交織成網。對貼壁分化後的NSCs進行免疫化學染色,其錶達神經元和膠質細胞的特異性蛋白。而未加入條件培養基的對照組,NSCs仍呈懸浮生長,未見大量細胞貼壁生長及分化。結論在完全去除BMSCs細胞的情況下,含有大鼠BMSCs分泌的細胞因子的條件培養基對NSCs的貼壁分化有顯著的促進作用。
목적관찰골수간충질간세포( BMSCs)조건배양기대신경간세포( NSCs)분화적영향。방법제취제3대대서BMSCs조건배양기,농축후가도NSCs배양기중,대소배양적NSCs진행형태학관찰화면역화학염색법감정。결과재가입대서BMSCs조건배양기적실험조,24 h내견현부생장적NSCs구개시첩벽생장。지제3천,소유현부세포구기본첩벽생장,대량세포종중파출,신출돌기。제7천시,신출세포교직성망。대첩벽분화후적NSCs진행면역화학염색,기표체신경원화효질세포적특이성단백。이미가입조건배양기적대조조,NSCs잉정현부생장,미견대량세포첩벽생장급분화。결론재완전거제BMSCs세포적정황하,함유대서BMSCs분비적세포인자적조건배양기대NSCs적첩벽분화유현저적촉진작용。
Objective To observe the effect of the conditioned medium from bone marrow mesenchymal stem cells ( BMSCs) on the differentiation of neural stem cells( NSCs) . Methods The morphology and immunocytochemistry profile of NSCs were investigated via culturing with the concentrated conditioned medium derived from the passage 3 BMSCs. Results In the conditioned medium of BMSC treated group, the growth of NSCs was converted from float-ing to adherence gradually within 24h. Till the 3rd day, most floating cells were adhered to the plastic and extended long processes which formed a network with the migrating cells on the 7 th day. By immunocytochemistry examina-tion, the cells differentiated from the adhering NSCs expressed the phenotypes of neurons and astrocytes. Neverthe-less, the NSCs without BMSC-CM was keeping floating growth in control group and observed adhering to plastic rarely. Conclusion Without the BMSCs themselves, the deliver factors secreted by BMSCs in the form of concen-trated conditioned medium promote the differentiation of NSCs.
