CAJ | 학술논문

目的探讨缺血预处理不同时间方案对肢体缺血再灌注损伤保护作用的差异性,选择合理的缺血预处理时间。方法40只SPF级SD大鼠随机分成5组：假手术组( A组,仅行开腹,分离腹主动脉不阻断)；缺血再灌注组(B组,夹闭腹主动脉缺血2 h后再灌注2 h)；C、D、E组：分别阻断腹主动脉1、5和10 min,再灌注1、5和10 min,重复3个循环后进行2 h缺血2 h再灌注。测定血清超氧化物歧化酶( SOD)、丙二醛(MDA)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)水平,观察各组氧化/抗氧化指标及炎症因子表达的差异性。结果D 组 SOD 活性升高、MDA含量下降,与B组比较差异有统计学意义( P<0.05)；E组改变更明显,和 D 组比较差异有统计学意义(P <0.05)。与B组比较,C、D、E组的IL-6、TNF-α明显升高,差异均有统计学意义(P<0.05)；E组的各炎症因子水平最高,与C组比较差异均有统计学意义( P<0.05)。结论预缺血时间与氧化损伤的保护作用在一定时间窗内呈平行关系,预缺血并无明显抗炎作用,过长时间预缺血甚至能导致全身性炎症反应；5 min/3个循环缺血预处理方案较为适宜。
목적탐토결혈예처리불동시간방안대지체결혈재관주손상보호작용적차이성,선택합리적결혈예처리시간。방법40지SPF급SD대서수궤분성5조：가수술조( A조,부행개복,분리복주동맥불조단)；결혈재관주조(B조,협폐복주동맥결혈2 h후재관주2 h)；C、D、E조：분별조단복주동맥1、5화10 min,재관주1、5화10 min,중복3개순배후진행2 h결혈2 h재관주。측정혈청초양화물기화매( SOD)、병이철(MDA)、백세포개소-6(IL-6)、종류배사인자-α(TNF-α)、백세포개소-10(IL-10)수평,관찰각조양화/항양화지표급염증인자표체적차이성。결과D 조 SOD 활성승고、MDA함량하강,여B조비교차이유통계학의의( P<0.05)；E조개변경명현,화 D 조비교차이유통계학의의(P <0.05)。여B조비교,C、D、E조적IL-6、TNF-α명현승고,차이균유통계학의의(P<0.05)；E조적각염증인자수평최고,여C조비교차이균유통계학의의( P<0.05)。결론예결혈시간여양화손상적보호작용재일정시간창내정평행관계,예결혈병무명현항염작용,과장시간예결혈심지능도치전신성염증반응；5 min/3개순배결혈예처리방안교위괄의。
Objective To investigate the relationship between different ischemic preconditioning protocols and the protective effects of hind limb ischemia/reperfusion injury in rats in purpose of choosing an optimal ischemic pre-conditioning scheme. Methods An experimental study was designed using 40 SD rats divided in five groups ( n=8). Group A: the sham group, laparotomy and separating the abdominal aorta without clamping for 240 minutes ( min) . Group B:ischemia/reperfusion,rats submitted to ischemia for 120 min and reperfusion for 120 min. Group C, D and E:animals submitted to three cycles of clamping and releasing the aorta for 1, 5 and 10 min respectively before being submitted to the ischemia/reperfusion procedure; the oxidative damage, inflammatory and protective effects among five groups were evaluated by measuring serum levels of SOD, MDA, IL-6, TNF-α, IL-10. Results Compared with group B,SOD activities were significantly higher in group D(P<0.05),SOD activities were sig-nificantly higher in group E compared with group D(P<0.05). Compared with group B, the level of IL-6, TNF-α increased obviously in group C, D, E(P<0.05). IL-6, TNF-α, IL-10 was the highest in group E among five groups ( P <0.05 ) . Conclusion The relationship between ischemia preconditioning time and anti-oxidation in hind limb ischemia/reperfusion injury seems paralleled. The inflammatory response accompanied with the ischemia preconditioning becomes serious with ischemia time prolonging. Preconditioning doesn’t show anti-inflammatory effect. Five min/three circulation ischemia preconditioning scheme is an optimal protocol.