中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
9期
2512-2516
,共5页
电针%贞芪扶正颗粒%肝纤维化%核转录因子-κB%转化生长因子-β1%结缔组织生长因子
電針%貞芪扶正顆粒%肝纖維化%覈轉錄因子-κB%轉化生長因子-β1%結締組織生長因子
전침%정기부정과립%간섬유화%핵전록인자-κB%전화생장인자-β1%결체조직생장인자
Electroacupuncture%Zhenqi Fuzheng Keli%Hepatic fibrosis%NF-κB%TGF-β1%CTGF
目的：探讨大鼠肝纤维化程度与自由基反应、核因子（ NF）-κB 活化、转化生长因子（ TGF）-β1mRNA 和结缔组织生长因子（ CTGF） mRNA表达的关系及电针联合贞芪扶正颗粒的干预作用。方法雄性大鼠300只，随机分为正常对照组、模型组、电针组、贞芪扶正颗粒组、针药联合组。以二甲基亚硝胺（ DMN）腹腔注射诱导大鼠肝纤维化模型，电针组造模同时给予电针足三里、关元、内关、三阴交、肝俞穴治疗，贞芪扶正颗粒组给予贞芪扶正颗粒灌胃，针药联合组则予上述电针和贞芪扶正颗粒治疗，共4w。造模4w后处死大鼠取肝脏组织标本，光镜观察肝脏组织的病理变化，并对肝脏组织标本进行病理评分；放射免疫法测定肝组织活性氧簇（ ROS）、抗氧化能力（ T-AOC）、超氧化物歧化酶（ SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性、脂质过氧化产物（LPO）含量及NF-κB活性，RT-PCR法检测肝组织中TGF-β1 mRNA及CTGF mRNA表达。结果与正常对照组比较，模型组大鼠的肝组织纤维化分期、ROS、LPO含量、NF-κB 活性、TGF-β1 mRNA 及 CTGF mRNA 表达显著增加（ P＜0.01），而T-AOC、SOD、GSH-Px、CAT活性明显下降（ P＜0.01）；与模型组比较，各治疗组大鼠的肝组织纤维化积分、ROS、LPO含量、NF-κB活性、TGF-β1 mRNA及CTGF mRNA表达显著下降，而血清T-AOC、SOD、GSH-Px、CAT活性明显上升（P＜0.01），联合治疗组上述指标优于其他治疗组（P＜0.05）。 NF-κB活性与TGF-β1 mRNA及CTGF mRNA呈正相关（r＝0.627，P＜0.05；r＝0.581，P＜0.05）。结论自由基反应、NF-κB 活化、TGF-β1及 CTGF与肝纤维化的严重程度密切相关，对肝纤维化发生、发展有协同作用，电针和贞芪扶正颗粒均能显著抑制自由基反应，降低NF-κB活性，下调TGF-β1和CT-GF mRNA的表达，从而减轻肝组织损害程度，且联合用药效果更好。
目的：探討大鼠肝纖維化程度與自由基反應、覈因子（ NF）-κB 活化、轉化生長因子（ TGF）-β1mRNA 和結締組織生長因子（ CTGF） mRNA錶達的關繫及電針聯閤貞芪扶正顆粒的榦預作用。方法雄性大鼠300隻，隨機分為正常對照組、模型組、電針組、貞芪扶正顆粒組、針藥聯閤組。以二甲基亞硝胺（ DMN）腹腔註射誘導大鼠肝纖維化模型，電針組造模同時給予電針足三裏、關元、內關、三陰交、肝俞穴治療，貞芪扶正顆粒組給予貞芪扶正顆粒灌胃，針藥聯閤組則予上述電針和貞芪扶正顆粒治療，共4w。造模4w後處死大鼠取肝髒組織標本，光鏡觀察肝髒組織的病理變化，併對肝髒組織標本進行病理評分；放射免疫法測定肝組織活性氧簇（ ROS）、抗氧化能力（ T-AOC）、超氧化物歧化酶（ SOD）、穀胱甘肽過氧化物酶（GSH-Px）、過氧化氫酶（CAT）活性、脂質過氧化產物（LPO）含量及NF-κB活性，RT-PCR法檢測肝組織中TGF-β1 mRNA及CTGF mRNA錶達。結果與正常對照組比較，模型組大鼠的肝組織纖維化分期、ROS、LPO含量、NF-κB 活性、TGF-β1 mRNA 及 CTGF mRNA 錶達顯著增加（ P＜0.01），而T-AOC、SOD、GSH-Px、CAT活性明顯下降（ P＜0.01）；與模型組比較，各治療組大鼠的肝組織纖維化積分、ROS、LPO含量、NF-κB活性、TGF-β1 mRNA及CTGF mRNA錶達顯著下降，而血清T-AOC、SOD、GSH-Px、CAT活性明顯上升（P＜0.01），聯閤治療組上述指標優于其他治療組（P＜0.05）。 NF-κB活性與TGF-β1 mRNA及CTGF mRNA呈正相關（r＝0.627，P＜0.05；r＝0.581，P＜0.05）。結論自由基反應、NF-κB 活化、TGF-β1及 CTGF與肝纖維化的嚴重程度密切相關，對肝纖維化髮生、髮展有協同作用，電針和貞芪扶正顆粒均能顯著抑製自由基反應，降低NF-κB活性，下調TGF-β1和CT-GF mRNA的錶達，從而減輕肝組織損害程度，且聯閤用藥效果更好。
목적：탐토대서간섬유화정도여자유기반응、핵인자（ NF）-κB 활화、전화생장인자（ TGF）-β1mRNA 화결체조직생장인자（ CTGF） mRNA표체적관계급전침연합정기부정과립적간예작용。방법웅성대서300지，수궤분위정상대조조、모형조、전침조、정기부정과립조、침약연합조。이이갑기아초알（ DMN）복강주사유도대서간섬유화모형，전침조조모동시급여전침족삼리、관원、내관、삼음교、간유혈치료，정기부정과립조급여정기부정과립관위，침약연합조칙여상술전침화정기부정과립치료，공4w。조모4w후처사대서취간장조직표본，광경관찰간장조직적병리변화，병대간장조직표본진행병리평분；방사면역법측정간조직활성양족（ ROS）、항양화능력（ T-AOC）、초양화물기화매（ SOD）、곡광감태과양화물매（GSH-Px）、과양화경매（CAT）활성、지질과양화산물（LPO）함량급NF-κB활성，RT-PCR법검측간조직중TGF-β1 mRNA급CTGF mRNA표체。결과여정상대조조비교，모형조대서적간조직섬유화분기、ROS、LPO함량、NF-κB 활성、TGF-β1 mRNA 급 CTGF mRNA 표체현저증가（ P＜0.01），이T-AOC、SOD、GSH-Px、CAT활성명현하강（ P＜0.01）；여모형조비교，각치료조대서적간조직섬유화적분、ROS、LPO함량、NF-κB활성、TGF-β1 mRNA급CTGF mRNA표체현저하강，이혈청T-AOC、SOD、GSH-Px、CAT활성명현상승（P＜0.01），연합치료조상술지표우우기타치료조（P＜0.05）。 NF-κB활성여TGF-β1 mRNA급CTGF mRNA정정상관（r＝0.627，P＜0.05；r＝0.581，P＜0.05）。결론자유기반응、NF-κB 활화、TGF-β1급 CTGF여간섬유화적엄중정도밀절상관，대간섬유화발생、발전유협동작용，전침화정기부정과립균능현저억제자유기반응，강저NF-κB활성，하조TGF-β1화CT-GF mRNA적표체，종이감경간조직손해정도，차연합용약효과경호。
Objective To explore the relationship between liver fibrosis degree and free radical reaction , NF-κB activation, TGF-β1 and CTGF mRNA expression in rats and the interventional effect of combining electroacupuncture and Zhenqi Fuzheng Keli on them .Meth-ods 300 male SD rats were randomly divided into five groups:normal control , model control , electroacupuncture , Zhenqi Fuzheng Keli and combination groups .The DMN was injected intraperitoneally for 4 weeks to induce liver fibrosis .At the same time of modeling , each rat in electroacupuncture group was treated with electroacupuncture of point Zusanli , Guanyuan, Neiguan, Sanyinjiao, Ganyu.While Zhenqi Fuzheng Keli was poured into gastric cavity in Zhenqi Fuzheng Keli group , and each rat was treated with above-mentioned puncture and Zhenqi Fuzheng Keli.The rats were killed after 4 weeks.The histomorphylogic structure of the liver tissue were observed under optical mi-croscope, and the damage degree of liver tissue was evaluated by a pathologic grading system .The levels of ROS, T-AOC, SOD, GSH-Px, CAT and LPO and NF-κB activity in liver tissue were determined by radioimmunoassay .The TGF-β1 and CTGF mRNA expression were measured with the methods of RT-PCR.Results Compared with normal control group , the fibrosis score, ROS and LPO levels, NF-κB ac-tivity, TGF-β1 and CTGF mRNA expression were increased obviously , the levels of T-AOC, SOD, GSH-Px and CAT were decreased obvi-ously in model group (P<0.01).Compared with model control group, the fibrosis score, ROS and LPO levels, NF-κB activity, TGF-β1 and CTGF mRNA expression were decreased obviously , the levels of T-AOC, SOD, GSH-Px and CAT were increased obviously in all treated groups (P<0.01), above-mentioned detecting indexes were better in combination group than those in other treated groups (P<0.05).There were obvious positive correlations among NF-κB activity, TGF-β1 and CTGF mRNA expression ( r1 =0.554, P<0.05; r2 =0.572, P<0.05).Conclusions Free radical reaction, activated NF-κB, TGF-β1 and CTGF are closely correlated to severity of hepatic fibrosis , there may be a synergetic interaction among free radical reaction , NF-κB, TGF-β1 and CTGF on the elevated pathogenesis of alcoholic hepat-ic fibrosis.Electroacupuncture and Zhenqi Fuzheng Keli can both inhibit free radical reaction , decrease NF-κB activity and downregulate TGF-β1 and CTGF mRNA expression obviously in rats with hepatic fibrosis to alleviate the severity of hepatic fibrosis and the effect of thera -peutic alliance is more better .
