CAJ | 학술논문

目的：探讨DcR3在预防博莱霉素大鼠模型发生肺纤维化中的作用。方法采用博莱霉素复制肺纤维化动物模型。分为正常对照组、博莱霉素组、博莱霉素＋DcR3组，给药时间2 w，各组分别于2、4、6、8 w处死大鼠6只，收集肺泡灌洗液的细胞和上清检测细胞因子含量。左肺组织冻存留作羟脯氨酸检测；右肺组织固定用作组织染色。结果博莱霉素＋DcR3组肺泡炎的程度表现为逐渐减轻的过程，炎症程度均低于 BL组（P＜0.05）；BL＋DcR3组肺纤维化程度无明显加重趋势，与对照组比较差异显著（P＜0.05），与BL组比较有显著性差异（P＜0.01）。结论 DcR3的早期应用，可减轻博莱霉素大鼠模型肺的的局部炎症，可能在一定程度上达到预防肺纤维化形成的目的。
목적：탐토DcR3재예방박래매소대서모형발생폐섬유화중적작용。방법채용박래매소복제폐섬유화동물모형。분위정상대조조、박래매소조、박래매소＋DcR3조，급약시간2 w，각조분별우2、4、6、8 w처사대서6지，수집폐포관세액적세포화상청검측세포인자함량。좌폐조직동존류작간포안산검측；우폐조직고정용작조직염색。결과박래매소＋DcR3조폐포염적정도표현위축점감경적과정，염증정도균저우 BL조（P＜0.05）；BL＋DcR3조폐섬유화정도무명현가중추세，여대조조비교차이현저（P＜0.05），여BL조비교유현저성차이（P＜0.01）。결론 DcR3적조기응용，가감경박래매소대서모형폐적적국부염증，가능재일정정도상체도예방폐섬유화형성적목적。
Objective To investigate the role of DcR 3 in preventing bleomycin rat model of pulmonary fibrosis .Methods The ble-omycin animal model of pulmonary fibrosis was established .The animal models were divided into normal control , bleomycin, bleomycin +DcR3 groups.The alveolar lavage fluid cells were cultured and supernatant , and the levels of cytokines were detected .Results The degree of alveolitis was reduced in Bleomycin+DcR3 group, which was lower than that of BL group (P<0.05);and the degree of lung fibrosis was not significantly increased trend in Bleomycin +DcR3 group,which had significant difference compared with that of BL and control groups (P<0.05, P<0.01).Conclusions DcR3 early application could reduce lung local inflammation in bleomycin rat model , achieve the pur-pose of prevention of pulmonary fibrosis in some extent .