中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
15期
2427-2433
,共7页
郑幸龙%向俊西%李建辉%吕毅
鄭倖龍%嚮俊西%李建輝%呂毅
정행룡%향준서%리건휘%려의
组织构建%血管内皮细胞%组织工程%血管化%内皮细胞%血管化策略%共培养%脱细胞支架%诱导多能干细胞
組織構建%血管內皮細胞%組織工程%血管化%內皮細胞%血管化策略%共培養%脫細胞支架%誘導多能榦細胞
조직구건%혈관내피세포%조직공정%혈관화%내피세포%혈관화책략%공배양%탈세포지가%유도다능간세포
tissue engineering%endothelial cells%induced pluripotent stem cells%tissue scaffolds
背景：构建有效的血管系统对于三维结构组织内细胞的存活和功能表达均具有决定性作用。因此，寻找一种合适的血管化策略已成为组织工程领域研究的热点和亟待解决的问题。<br> 目的：总结并讨论组织工程血管化的理论基础及研究进展。<br> 方法：检索 PubMed 数据库相关文章，检索时间为2003至2013年，检索词为“tissue engineering, vascularization, endothelial cel , scaffold”，并限定文章语言种类为英语，再从中筛选出与主题相关的部分文献，进行总结讨论。<br> 结果与结论：初检得到124篇文章，按主题相关性对文献进一步筛选，最终纳入41篇文章。血管化一直是组织工程领域关注的焦点和难点，国内外研究过多种血管化的策略，如复合生长因子、细胞共培养、生物反应器动态培养、人工支架或脱细胞支架等，但尚未公认一种有效的血管化策略实现与受体血管的功能性连接和体内细胞的长期存活。应用诱导多能干细胞与其他干细胞共培养，并通过添加生长因子、优化培养条件等方法在体内定向诱导分化，有望成为实现组织工程器官血管化的突破点。
揹景：構建有效的血管繫統對于三維結構組織內細胞的存活和功能錶達均具有決定性作用。因此，尋找一種閤適的血管化策略已成為組織工程領域研究的熱點和亟待解決的問題。<br> 目的：總結併討論組織工程血管化的理論基礎及研究進展。<br> 方法：檢索 PubMed 數據庫相關文章，檢索時間為2003至2013年，檢索詞為“tissue engineering, vascularization, endothelial cel , scaffold”，併限定文章語言種類為英語，再從中篩選齣與主題相關的部分文獻，進行總結討論。<br> 結果與結論：初檢得到124篇文章，按主題相關性對文獻進一步篩選，最終納入41篇文章。血管化一直是組織工程領域關註的焦點和難點，國內外研究過多種血管化的策略，如複閤生長因子、細胞共培養、生物反應器動態培養、人工支架或脫細胞支架等，但尚未公認一種有效的血管化策略實現與受體血管的功能性連接和體內細胞的長期存活。應用誘導多能榦細胞與其他榦細胞共培養，併通過添加生長因子、優化培養條件等方法在體內定嚮誘導分化，有望成為實現組織工程器官血管化的突破點。
배경：구건유효적혈관계통대우삼유결구조직내세포적존활화공능표체균구유결정성작용。인차，심조일충합괄적혈관화책략이성위조직공정영역연구적열점화극대해결적문제。<br> 목적：총결병토론조직공정혈관화적이론기출급연구진전。<br> 방법：검색 PubMed 수거고상관문장，검색시간위2003지2013년，검색사위“tissue engineering, vascularization, endothelial cel , scaffold”，병한정문장어언충류위영어，재종중사선출여주제상관적부분문헌，진행총결토론。<br> 결과여결론：초검득도124편문장，안주제상관성대문헌진일보사선，최종납입41편문장。혈관화일직시조직공정영역관주적초점화난점，국내외연구과다충혈관화적책략，여복합생장인자、세포공배양、생물반응기동태배양、인공지가혹탈세포지가등，단상미공인일충유효적혈관화책략실현여수체혈관적공능성련접화체내세포적장기존활。응용유도다능간세포여기타간세포공배양，병통과첨가생장인자、우화배양조건등방법재체내정향유도분화，유망성위실현조직공정기관혈관화적돌파점。
BACKGROUND:An efficient blood vessel system has a decisive effect on the survival and function expression of cells in three-dimensional tissues. Therefore it has been a hot research field in tissue engineering to find an appropriate vascularization strategy. <br> OBJECTIVE:To summarize and discuss the theory and research progress in vascularization strategies. <br> METHODS:Literature search was performed in PubMed database for English literatures published from 2003 to 2013. The key words are“tissue engineering, vascularization, endothelial cell, scaffold”in English. Then, the papers were further analyzed and reviewed in line with the theme. <br> RESULTS AND CONCLUSION:A total of 124 papers were searched. At last, 41 papers were selected according to the titles and objectives. Vascularization is the focus and pressing issue in tissue engineering field. There are many vascularization strategies, such as growth factor delivery, cellco-culture, dynamic-culture by bioreactor, scaffolds or decellularized scaffolds. But none of them is recognized as an effective strategy to achieve functional anastomosis with the host and sustain grafts survival for a long time in vivo. It wil be a big breakthrough in the future to co-culture pluripotent stem cells with other stem cells, combine with growth factors and optimize culture conditions for the differentiation in vivo.
