中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
18期
2867-2871
,共5页
董素明%常文凯%罗俊茜%薛佳杰%贾英伟%梁炳生
董素明%常文凱%囉俊茜%薛佳傑%賈英偉%樑炳生
동소명%상문개%라준천%설가걸%가영위%량병생
实验动物%组织构建%依达拉奉%肢体%缺血再灌注损伤%细胞凋亡%bcl-2%bax%氧自由基
實驗動物%組織構建%依達拉奉%肢體%缺血再灌註損傷%細胞凋亡%bcl-2%bax%氧自由基
실험동물%조직구건%의체랍봉%지체%결혈재관주손상%세포조망%bcl-2%bax%양자유기
reperfusion injury%lower extremity%free radical scavengers%cell death
背景:肢体缺血再灌注损伤中,氧自由基及细胞凋亡发挥重要的作用,通过抑制氧自由基生成及细胞凋亡,可减轻肢体缺血再灌注损伤。<br> 目的:验证依达拉奉在大鼠肢体缺血再灌注损伤方面的应用及疗效。<br> 方法:30只雌性SD大鼠,随机取20只,分别用自制气囊袖带环扎大鼠右后肢根部加压至40 kPa达到阻断血流,4h后松解形成再灌注,制作肢体缺血再灌注损伤模型。造模成功后随机分为2组,依达拉奉灌注组于再灌注前5 min由左股静脉注射依达拉奉3 mg/kg,模型组及剩余10只(正常组)于相同时间点给予等量的生理盐水。于再灌注24 h,取每组大鼠右胫前肌,用透射电镜观察其超微结构变化,并采用RT-PCR对每组大鼠胫前肌中bcl-2 mRNA、bax mRNA进行半定量检测并计算bcl-2/bax比值。<br> 结果与结论:①电镜结果显示:依达拉奉灌注组与模型组相比肌纤维较整齐,M 线、Z 线较清晰,线粒体肿胀减轻、其数量及嵴稍增多。②RT-PCR结果显示,缺血再灌注24 h后,右胫前肌bcl-2 mRNA的相对表达量以及bcl-2 mRNA与bax mRNA的比值:模型组显著低于依达拉奉灌注组(P<0.05),而bax mRNA的相对表达量模型组高于依达拉奉灌注组,且两组均高于正常组(P<0.05)。结果提示自由基清除剂依达拉奉可能通过改善线粒体等超微结构及促进bcl-2 mRNA、抑制bax mRNA的表达来减轻肢体缺血再灌注损伤,这可为肢体缺血再灌注损伤的治疗提供新的选择。
揹景:肢體缺血再灌註損傷中,氧自由基及細胞凋亡髮揮重要的作用,通過抑製氧自由基生成及細胞凋亡,可減輕肢體缺血再灌註損傷。<br> 目的:驗證依達拉奉在大鼠肢體缺血再灌註損傷方麵的應用及療效。<br> 方法:30隻雌性SD大鼠,隨機取20隻,分彆用自製氣囊袖帶環扎大鼠右後肢根部加壓至40 kPa達到阻斷血流,4h後鬆解形成再灌註,製作肢體缺血再灌註損傷模型。造模成功後隨機分為2組,依達拉奉灌註組于再灌註前5 min由左股靜脈註射依達拉奉3 mg/kg,模型組及剩餘10隻(正常組)于相同時間點給予等量的生理鹽水。于再灌註24 h,取每組大鼠右脛前肌,用透射電鏡觀察其超微結構變化,併採用RT-PCR對每組大鼠脛前肌中bcl-2 mRNA、bax mRNA進行半定量檢測併計算bcl-2/bax比值。<br> 結果與結論:①電鏡結果顯示:依達拉奉灌註組與模型組相比肌纖維較整齊,M 線、Z 線較清晰,線粒體腫脹減輕、其數量及嵴稍增多。②RT-PCR結果顯示,缺血再灌註24 h後,右脛前肌bcl-2 mRNA的相對錶達量以及bcl-2 mRNA與bax mRNA的比值:模型組顯著低于依達拉奉灌註組(P<0.05),而bax mRNA的相對錶達量模型組高于依達拉奉灌註組,且兩組均高于正常組(P<0.05)。結果提示自由基清除劑依達拉奉可能通過改善線粒體等超微結構及促進bcl-2 mRNA、抑製bax mRNA的錶達來減輕肢體缺血再灌註損傷,這可為肢體缺血再灌註損傷的治療提供新的選擇。
배경:지체결혈재관주손상중,양자유기급세포조망발휘중요적작용,통과억제양자유기생성급세포조망,가감경지체결혈재관주손상。<br> 목적:험증의체랍봉재대서지체결혈재관주손상방면적응용급료효。<br> 방법:30지자성SD대서,수궤취20지,분별용자제기낭수대배찰대서우후지근부가압지40 kPa체도조단혈류,4h후송해형성재관주,제작지체결혈재관주손상모형。조모성공후수궤분위2조,의체랍봉관주조우재관주전5 min유좌고정맥주사의체랍봉3 mg/kg,모형조급잉여10지(정상조)우상동시간점급여등량적생리염수。우재관주24 h,취매조대서우경전기,용투사전경관찰기초미결구변화,병채용RT-PCR대매조대서경전기중bcl-2 mRNA、bax mRNA진행반정량검측병계산bcl-2/bax비치。<br> 결과여결론:①전경결과현시:의체랍봉관주조여모형조상비기섬유교정제,M 선、Z 선교청석,선립체종창감경、기수량급척초증다。②RT-PCR결과현시,결혈재관주24 h후,우경전기bcl-2 mRNA적상대표체량이급bcl-2 mRNA여bax mRNA적비치:모형조현저저우의체랍봉관주조(P<0.05),이bax mRNA적상대표체량모형조고우의체랍봉관주조,차량조균고우정상조(P<0.05)。결과제시자유기청제제의체랍봉가능통과개선선립체등초미결구급촉진bcl-2 mRNA、억제bax mRNA적표체래감경지체결혈재관주손상,저가위지체결혈재관주손상적치료제공신적선택。
BACKGROUND:The oxygen free radicals and apoptosis play an important role in limb ischemia/reperfusion injury, so we can al eviate limb ischemia/reperfusion injury by inhibiting the production of oxygen free radicals and apoptosis. <br> OBJECTIVE:To discuss the application and effect of edaravone on limb ischemia/reperfusion injury in rats. <br> METHODS:Of the 30 female Sprague-Dawley rats, 20 rats were randomly selected to make models of limb ischemia/reperfusion injury by ligating the root of right lower limb with a self-made bal oon cuff at 40 kPa pressure to block blood flow for 4 hours and reperfusing. After success model establishment, they were randomly assigned to two groups. In the edaravone perfusion group, edaravone 3 mg/kg was injected via the left femoral vein at 5 minutes before reperfusion. In the model group and normal group (the remaining 10 rats), an equal volume of physiological saline was given at the same time point. At 24 hours after reperfusion, the right anterior tibial muscle of each group was removed and these ultrastructural changes were observed by transmission electron microscope. Bcl-2 mRNA and Bax mRNA of rat anterior tibial muscle of each group were semiquantitatively detected with the RT-PCR and the ratio of bcl-2/bax was calculated. <br> RESULTS AND CONCLUSION:(1)Electron microscope results:compared with the model group, the muscle fibers were neater;the M line and the N line were clearer;the swel ing of mitochondria was al eviated;the numbers of mitochondria and mitochondrial crista were also increased in the edaravone perfusion group. (2)RT-PCR results:At 24 hours after reperfusion, the relative expression of bcl-2 mRNA and the ratio of bcl-2 mRNA to bax mRNA in right anterior tibial muscle were lower in the model group compared with the edaravone perfusion group (P<0.05). However, relative expression of bax mRNA was greater in the model group than that in the edaravone perfusion group, which were both higher than the normal group (P<0.05). Results indicated that the free radical scavenger edaravone relieved limb ischemia/reperfusion injury by improving the mitochondrial ultrastructure and promoting expression of bcl-2 mRNA and inhibiting expression of bax mRNA, and could provide a new choice for the treatment of limb ischemia/reperfusion injury.