CAJ | 학술논문

目的：寻找人羊水中特异性表达的蛋白质。方法羊膜腔穿刺获取3份正常孕妇的羊水，同时采集其外周肘静脉血，分离血清。提取羊水及血清中的蛋白质，用双向凝胶电泳观察人羊水和血清中蛋白质表达情况，并选取羊水中表达量较血清中高2倍以上的蛋白斑点行基质辅助激光解吸/离子化飞行时间质谱分析。结果在pH 4～7、相对分子质量10～55 kDa区域里，羊水中约（613±29）个蛋白点，血清中约（785±64）个蛋白点。在羊水中表达但血清中不表达的蛋白点约50个，羊水中表达量较血清高2倍以上的蛋白点约50个。结论羊水中存在特异性表达蛋白质，其在妊娠相关生理、病理学中可能起重要作用。
목적：심조인양수중특이성표체적단백질。방법양막강천자획취3빈정상잉부적양수，동시채집기외주주정맥혈，분리혈청。제취양수급혈청중적단백질，용쌍향응효전영관찰인양수화혈청중단백질표체정황，병선취양수중표체량교혈청중고2배이상적단백반점행기질보조격광해흡/리자화비행시간질보분석。결과재pH 4～7、상대분자질량10～55 kDa구역리，양수중약（613±29）개단백점，혈청중약（785±64）개단백점。재양수중표체단혈청중불표체적단백점약50개，양수중표체량교혈청고2배이상적단백점약50개。결론양수중존재특이성표체단백질，기재임신상관생리、병이학중가능기중요작용。
Objective To explore the specific expression of proteomics in amniotic fluid (AF ).Methods Three samples were collected from normal AF by amniocentesis and peripheral venous blood sam-ples were collected simultaneously from which the serum was isolated. Proteins were extracted from both sam-ples and analyzed by two-dimensional gel electrophoresis (2-DE). The protein blots expressed in normal AF twice as high as that in serum were identified by matrix-assisted laser desorption ionizafion time-of-flight mass spectrometry.Results At pH 4-7 and within a molecular mass of 10-55 kDa,(613 ±29)protein blots were i-dentified in normal AF and (785 ±64)in serum. Approximately 50 protein blots were expressed in normal AF rather than serum. Roughly 50 blots were expressed in normal AF twice as high as that in serum. Conclusion Specific protein expression was observed in normal AF,which probably plays a vital role in pregnancy-related physiology and pathology.