CAJ | 학술논문

目的：探讨P-选择素对小鼠黑色素瘤肺转移的影响。方法将体外培养的B16黑色素细胞株通过小鼠的尾静脉注射到小鼠背景为C57/BL （C57）的P-选择素敲除的小鼠和C57小鼠的体内，建立转移瘤瘤模型，第21天断颈处死小鼠，计数转移到肺表面的结节的数目，HE染色进一步确定转移结节。结果 P-选择素敲除小鼠肺表面转移的肿瘤结节的数目明显比C57小鼠少，两者比较差异有统计学意义（P"0.01）。HE染色证实小鼠肺组织中有B16细胞的肺转移灶。结论 P-选择素缺失可能参与抑制了黑色素瘤的肺转移。
목적：탐토P-선택소대소서흑색소류폐전이적영향。방법장체외배양적B16흑색소세포주통과소서적미정맥주사도소서배경위C57/BL （C57）적P-선택소고제적소서화C57소서적체내，건립전이류류모형，제21천단경처사소서，계수전이도폐표면적결절적수목，HE염색진일보학정전이결절。결과 P-선택소고제소서폐표면전이적종류결절적수목명현비C57소서소，량자비교차이유통계학의의（P"0.01）。HE염색증실소서폐조직중유B16세포적폐전이조。결론 P-선택소결실가능삼여억제료흑색소류적폐전이。
Objective To study the effect of P-selectin on pulmonary metastasis of melanoma in mice. Methods B16 cells were intravenously injected into P-selectin knockout and C57/BL(C57)mice. Af-ter three weeks,the mice were sacrificed by cervical dislocation. Lung metastasis was observed by gross anato-my. HE staining of the metastatic tumor tissue sections was performed to confirm homology with B16 cells. The number of metastatic foci was counted. Results P-selectin knockout mice developed fewer metastatic foci on the lung surface than C57 mice(P ! 0.01). HE staining of the metastatic tumors confirmed their homology with B16 cells. Conclusion P-selectin loss may be involved in the inhibition of melanoma metastasis to the lung.