新医学
新醫學
신의학
NEW CHINESE MEDICINE
2014年
6期
359-363
,共5页
李天晓%元刚%叶丽君%孙健%黎曙霞
李天曉%元剛%葉麗君%孫健%黎曙霞
리천효%원강%협려군%손건%려서하
胃癌%棉酚衍生物%Bcl2抑制剂%细胞信号通路
胃癌%棉酚衍生物%Bcl2抑製劑%細胞信號通路
위암%면분연생물%Bcl2억제제%세포신호통로
Gastric carcinoma%Apogossypolone%Bcl2 inhibitor%Wnt6
目的：研究棉酚衍生物ApoG2诱导胃癌细胞凋亡作用及对Wnt6调控作用的影响。方法以人胃腺癌细胞系BGC823细胞为模型，检测ApoG2作用下BGC823细胞生长抑制、形态学变化和细胞周期变化，以及给药前、后细胞Bcl2、Bax和Bak mRNA表达水平和Wnt6蛋白表达水平的变化。结果 ApoG2对BGC823细胞的生长抑制呈浓度依赖性，给药后经Hoechst染色可观察到典型凋亡细胞，经PI染色用流式细胞仪可检测到明显sub-G1峰；Wnt6蛋白含量和Bcl2 mRNA含量随ApoG2浓度升高而降低，Bax和Bak mRNA含量随ApoG2浓度升高而升高。结论 ApoG2能明显抑制人胃癌细胞BGC823的生长，诱导细胞凋亡，可能通过抑制细胞Wnt6蛋白表达从而调控Bcl2蛋白家族的基因转录，继而引起细胞凋亡。
目的：研究棉酚衍生物ApoG2誘導胃癌細胞凋亡作用及對Wnt6調控作用的影響。方法以人胃腺癌細胞繫BGC823細胞為模型，檢測ApoG2作用下BGC823細胞生長抑製、形態學變化和細胞週期變化，以及給藥前、後細胞Bcl2、Bax和Bak mRNA錶達水平和Wnt6蛋白錶達水平的變化。結果 ApoG2對BGC823細胞的生長抑製呈濃度依賴性，給藥後經Hoechst染色可觀察到典型凋亡細胞，經PI染色用流式細胞儀可檢測到明顯sub-G1峰；Wnt6蛋白含量和Bcl2 mRNA含量隨ApoG2濃度升高而降低，Bax和Bak mRNA含量隨ApoG2濃度升高而升高。結論 ApoG2能明顯抑製人胃癌細胞BGC823的生長，誘導細胞凋亡，可能通過抑製細胞Wnt6蛋白錶達從而調控Bcl2蛋白傢族的基因轉錄，繼而引起細胞凋亡。
목적：연구면분연생물ApoG2유도위암세포조망작용급대Wnt6조공작용적영향。방법이인위선암세포계BGC823세포위모형，검측ApoG2작용하BGC823세포생장억제、형태학변화화세포주기변화，이급급약전、후세포Bcl2、Bax화Bak mRNA표체수평화Wnt6단백표체수평적변화。결과 ApoG2대BGC823세포적생장억제정농도의뢰성，급약후경Hoechst염색가관찰도전형조망세포，경PI염색용류식세포의가검측도명현sub-G1봉；Wnt6단백함량화Bcl2 mRNA함량수ApoG2농도승고이강저，Bax화Bak mRNA함량수ApoG2농도승고이승고。결론 ApoG2능명현억제인위암세포BGC823적생장，유도세포조망，가능통과억제세포Wnt6단백표체종이조공Bcl2단백가족적기인전록，계이인기세포조망。
Objective To investigate the apoptosis-inducing effect of ApoG2 on gastric carcinoma cells and its influence on Wnt6 expression. Methods Experiments were performed on human gastric carcino-ma cell line BGC823. Cell viability,cell morphology and cell cycle were detected after ApoG2 treatment. The mRNA Levels of Bcl2,Bax,Bak and Wnt6 protein were also examined. Results BGC823 cell growth was significantly inhibited by ApoG2 in a dose-dependent manner. Apoptotic cells were observed by fluorescence microscope using Hoechst staining after ApoG2 treatment. Cell cycle analysis of ApoG2 treated cells by flow cy-tometry displayed increased percentage of sub-G1 peak. Increased mRNA levels of Bax and Bak after ApoG2 treatment were observed,while both Bcl2 mRNA level and Wnt6 protein level were decreased. Conclusions ApoG2 can significantly inhibit the growth of human gastric carcinoma BGC823 cells and induce cell apoptosis.This effect is probably mediated by the suppression of Wnt6 protein which downregulates Bcl2 gene expression and upregulates Bax and Bak gene expression.
