农产品加工·学刊（中）
農產品加工·學刊（中）
농산품가공·학간（중）
AEM RODUCTS ROCESSING
2014年
4期
37-39,43
,共4页
向殿军%满丽莉%张春凤%张彩玲%张娣%殷奎德%曹天旭%王丽娜
嚮殿軍%滿麗莉%張春鳳%張綵玲%張娣%慇奎德%曹天旭%王麗娜
향전군%만려리%장춘봉%장채령%장제%은규덕%조천욱%왕려나
玉米秸秆%纤维素降解菌%16S rRNA%产酶条件
玉米秸稈%纖維素降解菌%16S rRNA%產酶條件
옥미갈간%섬유소강해균%16S rRNA%산매조건
corn straw%cellulose-degrading fungus%16S rRNA%enzyme-producing conditions
从富含腐烂玉米秸秆的土壤中分离筛选到1株能降解纤维素的细菌K01，经形态观察和16S rDNA序列分析，初步鉴定该菌属于美洲爱文氏菌。对该菌产酶条件进行优化，结果表明在接种种龄24 h，培养液初始pH值7，接种量3%，培养时间48 h的优化条件下，酶活力达1.58 IU/mL。该菌株对玉米秸秆降解效果较好，优化条件下7 d降解率达56.1%。
從富含腐爛玉米秸稈的土壤中分離篩選到1株能降解纖維素的細菌K01，經形態觀察和16S rDNA序列分析，初步鑒定該菌屬于美洲愛文氏菌。對該菌產酶條件進行優化，結果錶明在接種種齡24 h，培養液初始pH值7，接種量3%，培養時間48 h的優化條件下，酶活力達1.58 IU/mL。該菌株對玉米秸稈降解效果較好，優化條件下7 d降解率達56.1%。
종부함부란옥미갈간적토양중분리사선도1주능강해섬유소적세균K01，경형태관찰화16S rDNA서렬분석，초보감정해균속우미주애문씨균。대해균산매조건진행우화，결과표명재접충충령24 h，배양액초시pH치7，접충량3%，배양시간48 h적우화조건하，매활력체1.58 IU/mL。해균주대옥미갈간강해효과교호，우화조건하7 d강해솔체56.1%。
A cellulose-decomposing fungus strain is isolated from soil containing rich rotten corn straw. Based on morphology and 16Sr DNA sequence analysis, it is initially classified into the genus of Penicillium. And the optimum conditions for CMCase production are determined as follows: seed age of 24 hours, initial pH value of medium of 7, inoculum amout of 3%, culture time of 48 hours. Under the optimal conditions, the CMCase activity reaches 1.58 IU/mL. The strain K01 effectively degrades the corn straw, and the degradation rate reaches 56.1%in seven days.
